ITP患者NK细胞Fasl、Caspases-3和Caspases-8凋亡相关蛋白的表达和意义

Expression and Clinical Significance of Apoptosis-Related Protein Fasl,Caspase-3 and Caspases-8 in Peripheral Blood NK Cells of Patients with Idiopathic Thrombocytopenic Purpura

目的研究NK细胞Fasl、Caspases-3和Caspases-8凋亡相关蛋白在特发性血小板减少性紫癜(ITP)患者NK细胞的表达及其意义。方法选取30例初发ITP患者(ITP组),30名健康志愿者(对照组),采用流式细胞术检测两组外周血NK细胞(CD3-CD16+56+)Fasl,Caspases-3和Caspases-8凋亡相关蛋白的表达。结果与健康对照组相比,ITP患者组NK细胞表面Fasl表达率增加[(5.9±5.5)%](P<0.05),细胞质中活化Caspases-3和Caspases-8的表达率明显增加(P<0.05)。与治疗前相比,ITP患者组治疗后NK细胞表达活化Caspases-3和Caspases-8明显下降(P<0.05)。结论ITP患者外周血NK细胞Fasl、Caspases-3和Caspases-8明显增加,激素治疗能干预Caspases-3和Caspases-8的表达水平,提示ITP患者外周血NK细胞的Fasl及Caspases-3和Caspases-8信号通路在ITP发生机制中起一定作用。

Objective To study the expression and clinical significance of apoptosis-related protein （Fasl, Caspase-3 and Caspase-8） in peripheral blood NK cells of patients with idiopathic thrombocytopenic purpura（ITP）. Methods Collecting pe- ripheral blood from ITP patients （ n = 30 ） and health Volunteers （ n = 30 ）. The expression of apoptosis-related proteins Fasl, Caspases-3 and Caspases-8 in NK cells was detected by flow cytometry （ FCM ）. Results The expression of Fasl, activated Caspase-3 and activated Caspase-8 of NK cell in patients with ITP group increased as compared with the healthy controls （ P 〈 0.05 ） ;The expression of Caspase-3 and easpases-8 in NK in patients with ITP was significantly lower as compared with the levels before treatment（ P 〈 0.05 ）. Conclusion The expression of Fasl, Caspase-3 and Caspase-8 was significantly increased in pe- ripheral NK cells of patients with ITP; hormone therapy may interfere with the expression of Caspase-3 and Caspase-8. Fasl, Caspase-3 and Caspase-8 signaling pathway may play an impartment role in the mechanism of ITP.