miR-29a在宫颈癌组织中的表达及其意义

Expression and Significance of MiR-29a in Cervical Cancer Tissues

目的探讨miR-29a在宫颈癌组织中的表达及与宫颈癌临床病理因素之间的关系。方法用茎-环实时荧光定量PCR分析56例宫颈癌组织和20例正常宫颈组织中miR-29a的表达情况,分析miR-29a的表达与宫颈癌常用临床病理指标的关系。结果茎-环实时荧光定量PCR能特异扩增miR-29a。miR-29a在宫颈癌组织中的表达量明显低于正常宫颈组织(P<0.01)。miR-29a在Ⅱa期宫颈癌组织中的表达量明显低于Ⅰa期和Ⅰb期(P<0.01)。miR-29a的表达和年龄、肿瘤大小、肿瘤类型和病理分级无关(P>0.05)。结论 miR-29a是宫颈癌的重要标记和靶向治疗的潜在靶点。

Objective To investigate the expression of miR-29a and its association with clinicopathologic features in cervical cancer. Methods Expression of miR-29a in 56 cervical cancer and 20 normal control tissues were examined by real-time RT- PCR. The relationship between miR-29a expression and various clinicopathological characteristics in cervical cancer were ana- lyzed. Results The miR-29a was specifically amplified by real-time RT-PCR. Its expression in cervical cancer tissues was signif- icantly lower than that in normal cervical tissues（ P 〈 0.01 ）. The expression of miR-29a in FIGO stage ][ a was significantly low- er than that in stage Ia and stage Ib（P 〈0.01 ）. The level of miR-29a is not relevant with patient age,tumor size,general type and pathological grading of cervical cancer （ P 〉 0.05 ）. Conclusion The miR-29a is a valuable marker and may be a potential therapeutic target of cervical cancer.