二十二碳六烯酸联合5-氟尿嘧啶对人肝癌细胞HepG2耐药相关蛋白表达的影响

Effect of docosahexaenoic acid plus 5-fluorouracil on the drug-resistance associatedprotein of HepG2

目的研究二十二碳六烯酸(DHA)联合5氟尿嘧啶(5-FU)对人肝癌细胞HepG2耐药相关蛋白ERK、JNK及p38的表达。方法 Western blot检测5-FU5μg/mL,或5-FU5μg/mL联合DHA30μg/mL作用HepG2细胞48h后ERK、JNK及p38蛋白表达。结果与单用5-FU组比较,5-FU联合DHA组对耐药相关蛋白的影响,p-ERK明显受到抑制,而p-JNK表达显著增加,p-p38表达无差别。结论 DHA对肝癌细胞耐药相关蛋白的调控可能通过抑制ERK、激活JNK信号通路来增强5-FU的细胞毒活动,发挥增敏化疗药物的作用。

Objective To study the expression of drug-resistance associated protein, such as ERK, JUK and P38, in hepatocellular carcinoma HepG2 cells treated with docosahexaenoic acid and 5-fluorouracil. Methods The expression of JNK, ERK and p38 were analyzed in HepG2 treated with 5-FU or 5-FU in combination with DHA for 48 hours by western blot. Results When compared with the group only treated with 5-FU, samples from the group treated with 5-FU and DHA showed different expression level of drug- resistance associated-proteins. Down regulation of p-ERK, up regulation of p-JNK and no change of p-p38 could be found in the latter group. Conclusion With inhibiting the p-ERK and activating p-JNK, DHA could regulate hepatocellular carcinoma cells to significantly enhance the cytotoxic activity of 5-FU. So that it could play a role in the sensitization of chemotherapy drugs.