1202例疑似生殖器疱疹患者HSV基因检测结果分析

Analysis of genetic detecting in 1202 outpatients with suspected herpes simplex virus infection

目的了解广州新塘地区疑似生殖器疱疹患者单纯疱疹病毒(HSV)感染情况。方法用PCR-荧光探针法(FQ-PCR)对我院门诊1202例疑似生殖器疱疹患者进行HSV-I/II基因检测。结果在1202例生殖器疱疹疑似患者中,检测出HSV-I型阳性患者8例,阳性率为0.67%(8/1202);HSV-II型阳性患者282例,阳性率为23.46%(282/1202),总计单纯疱疹病毒阳性患者290例,阳性率24.13%(290/1202)。HSV-I型感染率与HSV-II型感染率比较,差异具有统计学意义(P<0.01)。290例单纯疱疹病毒感染患者中男性患者193例,阳性率24.62%(193/748),女性患者97例,阳性率23.21%(97/418),男性与女性的感染率比较,差异不具有统计学意义(P>0.05)。结论①广州新塘地区疑似生殖器疱疹患者HSV检出率较高近1/4,感染率有型别差异,以HSV-II型为主,提示临床需重视对疑似生殖器疱疹病毒患者进行HSV检测,避免误诊漏诊。②HSV感染率无性别差异。③FQ-PCR在HSV检测中具有简便、灵敏度高、快捷等特点,具有良好的推广、应用前景。

Objective To investigate the situation of patients with genital suspected herpes simplex virus （HSV） infection in Xintang, Guangzhou. Methods To test herpes simplex virus （HSV-I/II） of 1202 cases of outpatients by fluorescence quantitative polymerase chain reaction technology （FQ-PCR）. Results The positive rate of 1202 casas of outpatients were 0.67% （8/1202） with HSV-I and 23.46% （282/1202） with HSV-II. The total positive rate of HSV infection were 24.13% （290/1202）. There was significant difference between the positive detection rate of HSV-I and HSV-II （P〈0.01）. The positive rate of HSV in male and female patients were 24.62% （193/748） and 23.21% （97/418）, respectively. There was no significant difference of the positive detection rate between male and female （P〉0.05）. Conclusion （1） The detection rate of HSV in the outpatients with suspected genital herpes HSV infection was high in Xintang area, which nearly a quarter. The infection rate was difference in different types of HSV, and the main type was HSV-II. The result illustrated that clinical diagnosis should pay attention to detect HSV in outpatients with suspected genital herpes virus in order to avoid misdiagnosis. （2） There was no difference of HSV infection between male and female. （3） The features of FQ-PCR were simplicity, high sensitivity, fast in detection of HSV and it is worth for clinical application.