乌贼内脏酶解多肽的制备和抗氧化活性研究

Study on Preparation and Antioxidant Activity of Enzymatic Hydrolysis Peptide from Squid Organ

[目的]探讨乌贼内脏酶解多肽的制备和抗氧化活性。[方法]以乌贼内脏为原料,用胰蛋白酶对其进行水解,采用单因素和正交试验方法研究酶解温度、酶解时间、加酶量和pH对水解度和抗氧化性的影响;通过检测羟自由基清除率、DPPH清除率和还原力评价不同分子量水解物多肽的抗氧化性。[结果]当酶解温度为35℃,时间为5 h,加酶量为5 000 U/g,pH为7.5时,水解度最大;当酶解温度为40℃、时间为7 h、加酶量为4 500 U/g,pH为8.0时,水解物对羟自由基清除率最大。超滤后不同分子量多肽对羟自由基的清除率为3～5 kD>3 kD以下>5～10 kD;DPPH清除率:3～5 kD>3 kD以下>5～10 kD;还原力:3 kD以下>3～5 kD>5～10 kD。[结论]乌贼内脏酶解多肽具有较好的抗氧化活性,不同分子量多肽的活性不同。

[ Objective] To discuss the preparation and antioxidant activity of enzymatic hydrolysis peptide of squid organ. [ Method ] Peptide was extracted from squid organ by Trypsin, the effects of enzymolysis temperature, time, enzyme dose and pH on degree of hydrolysis and antiox- idant activity were studied through single factor and orthogonal experiment. Different molecular weight peptide oxidation resistance was evaluated by testing the scavenging activity of hydroxyl free radicals, and i＇educing power. [ Result] Degree of hydrolysis reached to maximum under the conditions of enzymolysis temperature 35 ℃, enzymolysis time 5 h, total enzyme dose 5 000 U/g and pH 7.5. The scavenging activity of hydroxyl free radicals reached to maximum under the conditions of enzymolysis temperature 40 ℃, enzymolysis time 7 h, total enzyme dose 4 500 U/g, and pH 8.0. The peptides was fractionated according to molecular weights （MW） using three different molecular weight cut-off membranes （ 10, 5 and 3 kDa） and were separated to three fractions, 3 -5 kD with the highest hydroxyl free radicals scavenging activity and 〈 3 kD with the high- est reducing power. [ Conclusion] Hydrolysis peptide of squid organ has a good antioxidant activity and differed in molecular weight.