不同磷浓度条件下三氯生对斜生栅藻的毒性影响研究

Toxicity Effects of Triclosan on Scenedesmus obliqnus at Different Phosphorus Concentrations

[目的]研究不同磷浓度条件下,三氯生对斜生栅藻的毒性影响。[方法]测定不同磷浓度条件(0、2.5、50.0 mg/L)不同浓度三氯生(50、200μg/L)对斜生栅藻生长、叶绿素荧光特性、生理生化指标的影响,从3个方面研究不同磷浓度条件下三氯生对斜生栅藻的毒性影响。[结果]在3种磷浓度条件(0、2.5、50.0 mg/L)下,三氯生对斜生栅藻的生长抑制表现出剂量-效应关系,斜生栅藻叶绿素荧光特性指标Yield、Fv/F0、NPQ受到不同程度的影响。斜生栅藻抗氧化系统酶(SOD、CAT)及磷代谢相关酶(ACP、AKP)活性亦发生相应变化。[结论]三氯生对斜生栅藻的毒性影响与环境中磷浓度变化有一定相关性。

[ Objective ] The research aimed to analyze the toxicity effects of triclosan to Scenedesmus obliqnus at different phosphorus concentra- tions. [ Method] Growth inhibition effect, chlorophyll fluorescence characteristics and relative enzyme activities were measured at different phos- phorus concentrations （0, 2.5, 50.0 mg/L） and triclosan concentrations （0, 50,200 μg/L）. [ Result] Under three phosphorus concentrations （0, 2.5, 50.0 mg/L）, growth inhibition effect of triclosan on Scenedesmus obliquus showed dose-effect relationship, chlorophyll fluorescence characteristics Yield,FJF0, NPQ of Scenedesmus obliqnus were affected to different extents. Activities of anti-oxidative enzymes （ SOD, CAT）, and phosphorus metabolism related enzymes （ACP, AKP） also changed accordingly. [ Conclusion ] Toxicity effect of triclosan on Scenedesmus obliquus is correlative with phosphorus concentration in the environment.