CD4^+CD25^+调节性T细胞检测在急性髓细胞白血病中的临床意义

Clinical significance of CD4^+CD25^+ regulatory T cells detection in acute myelocytic leukemia

目的:探讨CD4+CD25+调节性T细胞检测在急性髓细胞白血病(AML)中的临床意义。方法:选取AML患者41例和体检健康者16例,用流式细胞仪检测CD4+CD25+调节性T细胞占CD4+T细胞的比例。结果:AML患者外周血的CD4+CD25+调节性T细胞表达明显高于对照者[(3.88±1.99)%∶(1.96±1.66)%,P<0.01]。AML组内,化疗耐药者(15例)的CD4+CD25+调节性T细胞表达显著高于化疗敏感者(14例)[(3.90±1.89)%∶(2.00±1.65)%,P<0.01]。14例化疗敏感者中,缓解后复发者(4例)的CD4+CD25+调节性T细胞表达显著高于持续缓解者(10例)[(3.95±1.86)%∶(2.02±1.55)%,P<0.05]。结论:CD4+CD25+调节性T细胞可能诱导免疫耐受,从而导致AML细胞的恶性增殖,影响白血病化疗的疗效,也可能是引起缓解患者复发的原因之一。

Objective：To explore the clinical significance of CD4＋CD25＋ regulatory T cells detection in acute myelocytic leukemia（AML）. Method：The percentage of CD4＋CD25＋ regulatory T cells in CD4＋ T cells was detected in 41 AML and 16 normal controls by flow cytometry. Result：CD4＋CD25＋ regulatory T cells expression in AML patients was significantly higher than that in normal controls（P0.01）.CD4＋CD25＋ regulatory T cells expression in 15 patients with chemotherapy tolerance was significantly higher than that in 14 patients with chemotherapy sensitivity（P0.01）.CD4＋CD25＋ regulatory T cells expression in 4 patients with recurrence was significantly higher than that in 10 patients with sustained remission（P0.05）. Conclusion：CD4＋CD25＋ regulatory T cells may induce immune tolerance and lead to acute leukemia cell proliferation and influence the curative effect of leukemia chemotherapy.CD4＋CD25＋ regulatory T cells may be one of the reasons which resulted in the recurrence of patients after remission.