金标法和直接免疫荧光法检测呼吸道合胞病毒的比较

Comparison of Immuno-gold Method and Direct Immunofluorescence to Detect Respiratory Syncytial Virus

目的 金标法和直接免疫荧光法检测呼吸道合胞病毒的方法比较.方法同时用金标法和直接免疫荧光法检测350例儿童患者的鼻咽拭子中呼吸道合胞病毒.结果 350例中,直接免疫荧光法检出173例阳性,阳性率为49.4%;金标法检出121例阳性,阳性率为34.6%,两种检测方法灵敏度比较差异有统计学非常显著性意义（P＜0.01）,但两种检测方法的检测结果一致性检验,kappa=0.702,基本一致.结论 直接免疫荧光法比金标法的灵敏度高,两种检测方法的检测结果基本一致.

Objective Comparison of immuno-gold method and direct immunofluoreseence to detect respiratory syncytial virus （RSV）. Methods 350 cases of nasopharynx swabs of pediatric patients were detected by immuno-gold method and direct immunofluorescence. Results 173 cases were positive in 350 cases by direct immunofluorescence assay. The rate of positive was 49.4; by direct immunofluorescence assay. 121 cases were positive by immuno-gold assay. The rate of positive was 49, 4% by immuno-gold assay. There was very significant difference between immuno-gold method and direct immunofluorescence. The sensitivity of the imrnuno-gold method was only 69.9% of the direct immunofluoescence. Both had the same specificity. Conclusion The immuno-gold method is simpler and more rapid than the direct immunofluorescence method,but it＇s sensitivity was lower than the latter. Both had the same specificity. Immuno-gold method can be used as the detection of respiratory syneytial virus in out-patient test and primary hospitals.