西安地区女性生殖道感染HPV基因型调查分析

Investigation and Survey on Human Papillomavirus Subtypes Infection in the Lower Genital Tract of Women in Xi＇ an City

目的 调查西安地区女性生殖道感染HPV基因型分布状况,了解不同亚型HPV在女性中的感染情况及流行病学意义.方法 对2012年6月～2013年2月在陕西省人民医院妇科门诊及住院患者阴道分泌物标本采用PCR-反向斑点杂交法检测19个HPV基因型,包括低危型（HPV6,11和43）和高危型（HPV16,18,31,33,35,39,45,51,52,53,56,58,59,66,68和CP8304亚型）.结果 1 786例样本中检出HPV阳性者514例,检出率为28.8%,HPV阳性者中低危型占30.9%,高危型占39.7%,两型和两型以上混合感染占29.4%;1 786例样本中感染率最高的为低危HPV6型（9.1%）,其他型别分别为HPV16（5.5%）,HPV11（3.9%）,HPV52（3.8%）,HPV58（2.7%）,HPV66（2.4%）,HPV53（2.0%）,HPV68（1.8%）,HPV51（1.6%）,HPV39（1.4%）,HPV33（1.2%）,HPV31（1.0%）,HPV-CP8304（1.0%）,HPV18（0.7%）,HPV43（0.7%）,HPV59（0.5%）,HPV56（0.11%）,HPV35（0.10%）和HPV45（0.09%）.结论 西安地区女性生殖道感染HPV基因分布有区域特点,HPV检测具有重要的流行病学意义.

Objectives Our purpose of study was to investigate HPV subtypes infection in lower genital tract of female patients and its epidemiological significance in Xi＇an city. Methods A total of 1786 cases of female patients from June 2012 to February 2013 were detected for 19 types of HPV DNA by PCR-RDB including low risk subtypes （HPV6,11 and 43） and high risk subtypes （HPV16,18,31,33,35,39,45,51,52,53,56＇58,59,66,68 and CP8304）. Results The total infection rate of HPV was 28.8% （514/1786） among which the positive rate for low risk types was 30. 9% ,that for high risk ones was 39.7% and that for two or more ones was 29.4%. The 19 subtypes were detected including 9.1% for HPV6,which was the most common type,and the other common types,in descending order of frequency, were HPV16 （5.5 %）, HPVll （3.9%）,HPV52 （3. 8%）,HPV53 （2.0%）,HPV58 （2.7%）,HPV66 （2. 4%）,HPV68 （1.8%）,HPV51 （1.6%）,HPV39 （1.4%）,HPV33 （1.2%）,HPV31 （1.0%）,HPV-CP8304 （1.0%）,HPV18 （0. 7%）,HPV43 （0. 7%）,HPV59 （0. 5%）, HPV56 （0. 11%）, HPV35 （0. 10%） and HPV45 （0. 09;）. Conclusion The distribution of HPV subtypes in lower genital of women had respective regional character and detection of HPV has an important epidemiological significance.