RKIP的表达与肺癌侵袭转移的相关性

Correlation of RKIP expression and invasion and metastasis of lung cancer

目的探讨Raf激酶抑制蛋白(RKIP)在肺癌组织中的表达及其与肺癌侵袭转移的相关性。方法采用RT-PCR和Western blot方法检测95例肺癌患者的肺癌组织及其癌旁正常肺组织中RKIP的表达,分析其与肺癌临床病理学特征的关系。结果肺癌组织RKIP mRNA阳性表达率和蛋白表达量均低于正常肺组织(48.42%vs.77.89%和0.598±0.124vs.0.623±0.116),肺癌中、低分化组均低于高分化组[(39.47%、20.00%vs.66.67%)和0.318±0.117、0.104±0.005vs.0.623±0.116),伴有淋巴结转移组低于无淋巴结转移组(31.71%vs.61.11%和0.309±0.112vs.0.737±0.175),生存期<2年者低于生存期≥2年者(34.78%vs.61.22%和0.302±0.107vs.0.742±0.225)(P<0.05)。结论 RKIP的低表达可能与肺癌的发生及侵袭转移有关。

Objective To investigate the expression of Raf kinase inhibitor protein（RKIP） in lung cancer and its correlation with the invasion and metastasis of lung carcer. Methods The expressions of RKIP in lung cancer（group A） and tumor-adjacent normal lung tissues（group B） were detected by RT-PCR and Western blot in 95 patients with lung cancer. The relationship of RKIP expression with elinicopathologic features was analyzed. Results The RKIP mRNA positive expression rate and protein expression were lower in group A than those in group B[（48. 42% vs. 77. 89%） and （0. 598±0. 124 vs. 0. 623±0. l16）],which in group A were lower in moderately and poorly differentiated groups than those in well differentiated group[（39.47M, 20.00% vs. 66.67%） and （0. 318±0. 117,0. 104±0. 005 vs. 0. 623±0. ll6）],lower in the cases with lymphatic metastasis than those without[（31.71% vs. 61.11%） and （0. 309_＋0. 112 vs. 0. 737±0. 175）],and lower in the cases with survival time 〈2 years than those ≥2 years [（34. 78%vs. 61.22%） and （0. 302±0. 107 vs. 0. 742 ± 0. 225）] （P〈0. 05）. Conclusion The low expression of RKIP may be correlated with carcinogenesis, invasion and metastasis of lung cancer.