摘要
目的研究高密度脂蛋白(HDL)对凝血酶刺激的人血小板α颗粒膜糖蛋白(CD62P或P-选择素)、溶酶体膜糖蛋白(CD63)表达的影响。方法 3组等体积手工制备的洗涤血小板分别加入0.5、1和10U/mL的凝血酶(低、中、高凝血酶组),另外3组同样含量的凝血酶加入37℃孵育15min的HDL,分别为低、中、高复合组。同时37℃环境下孵育10min,以流式细胞仪检测各组CD62P、CD63的表达。结果低、中、高复合组CD62P表达率分别为11.55%±1.34%,17.19%±0.17%,19.79%±0.32%,均低于相应凝血酶组的18.14%±1.50%,26.24%±0.77%,80.38%±5.66%(均P<0.01),低、中、高复合组CD63表达也低于相应的凝血酶组即(2.92%±0.22%、4.20%±0.98%,5.12%±0.09%vs8.09%±0.48%、14.15%±1.39%、24.48%±1.71%,均P<0.01)。低、中复合组血小板CD62P、CD63抑制率均低于高复合组(均P<0.05)。结论在体外,HDL抑制了凝血酶刺激的血小板α颗粒膜糖蛋白和溶酶体膜糖蛋白的表达。
Objective To investigate the effects of high density lipoprotein （HDL） on thrombin-activated platelet al- pha-granulemembrane protein （CD62P） and lysosome intact membrane protein （CD63） expressions in vitro. Methods The equivalent volume of washed platelets prepared by hand was preincubated with HDL （1 g/L） in 37 ℃water for 15 minutes, which was then stimulated with different concentrations of thrombin （0.5 U/mL, 1 U/mL and 10 U/mL） for 10 minutes in wa- ter of 37 ℃. Meanwhile another three groups of washed platelets were incubated with thrombin （0.5 U/mL, 1 U/mL and 10 U/ mL） for 10 minutes, respectively. The CD62P and CD63 from each sample were analyzed by flow cytometry （FCM）. Results The CD62P positive rates of HDL-preincubated groups were significantly lower than those of different concentrations of thrombin groups （0.5 U/mE,1 U/mL and 10 U/mL） in the absence of HDL （11.55%±1.34% vs 18.14%±1.50%, 17.19%± 0.17% vs 26.24%±0.77% and 19.79%±0.32% vs 80.38%±5.66%, P 〈 0.01）. Meanwhile, The CD63 positive rates of HDL-preineubated groups were also significantly lower than those of thrombin-treated （0.5 U/mL, l U/mL and 10 U/mL） groups without HDL, namely,2.92%±0.22% vs 8.09%.48% （P 〈 0.001）, 4.20%±0.98% vs 14.15%±l.39% （P 〈 0.001） and 5.12% ±0.09% vs 24.48% ± 1.71% （P 〈 0.01）. Conclusion HDL inhibits the expression of CD62P and CD63 on throm- bin-stimulated platelets in vitro.
出处
《天津医药》
CAS
北大核心
2013年第7期647-649,I0001,共4页
Tianjin Medical Journal
基金
全军"十一五"科研基金项目(项目编号:06G145)
