轻度认知障碍进展为阿尔茨海默病患者外周血促炎细胞因子浓度变化的研究

Research on the relationship between proinflammatory cytokines and cognitive function in patients withmild cognitive impairment

目的研究轻度认知障碍（MCI）患者外周血中促炎细胞因子浓度与认知功能的动态变化关系。方法搜集MCI患者70例，评估患者的认知功能，动态随访采集患者静脉血，利用酶联免疫吸附试验检测促炎细胞因子肿瘤坏死因子α（TNF-α）、白细胞介素（IL）-1α、IL-1β和IL-6的浓度。结果70例MCI患者，符合遗忘型MCI（aMCI）标准的50例，其中19例进展为阿尔茨海默病（AD）。19例进展为AD的aMCI患者疾病进展前与进展后细胞因子IL-1β[（40．5±7．7和38．6±7．3）μg/L，t=3．537、P=0．002]，IL-6[（70．4±24．3和53．6±20．5）μg/L，t=2．229、P=0．0393和TNFd[（58．6±13．5和50．3±17．1）μg/L，t=2．226、P=0．0393差异有统计学意义。结论MCI患者进展为AD后IL-1β、IL-6和TNF-α含量均降低。

Objective To investigate the relationship between proinflammatory cytokines and cognitive function in patients with mild cognitive impairment （MCI）. Methods From May 2007 to May 2009, 70 patients （aged ≥ 60 years） with MC1 were collected. Among them, 50 cases were amnestic MCI, and 19 cases developed into AD. The cognitive function was assessed, and all patients were followed up. The venous blood samples were obtained and proinflammatory cytokines （IL-1α, IL-1β, IL-6, TNF-α） were detected by using enzyme-linked immunosorbent assay. Results There were differences in the levels of proinfiammatory cytokines between patients with aMCI and patients with Alzheimer＇s disease （AD） [IL-1α, （40.5±7.7） μg/L vs. （38.6±7.3） μg/L; IL-6, （70.4±24.3）μg/L vs. （53.6±20.5）μg/L;TNF-α,, （58.6±13.5） μg/Lvs. （50.3±17.1） μg/L;t=3.537, 2. 229, 2. 226, P=0. 002, 0. 039, 0. 039, respectively3. Conclusions MCI is a preclinical state of AD. The cognitive function damage of MCI patients are different from that of AD patients, and the immune status of MCI patients is also changed.