对碘乙酸诱导猫视网膜光感受器退行性损伤的功能评估

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摘要已知碘乙酸可诱导多种动物产生急性光感受器退行性变.本研究旨在探索采用IAA完全去除猫视网膜光感受器并形成全盲动物模型.经耳缘静脉注射20mg/kgIAA,6个月后进行瞳孔对光反射、视网膜电图、行为学及组织化学等项评估.结果显示,在注射IAA6个月后,动物丧失全部光感受器细胞并且视觉功能完全消失呈全盲.经耳缘静脉注射高浓度IAA可快速诱导猫视网膜产生稳定的光感受器退行性变模型.可为采用基因和细胞疗法治疗视网膜晚期疾病和恢复视觉功能的各类生物技术提供一个有用的动物模型.

作者南燕张钦任超然黄鑫高洁黎晓新濮鸣亮

机构地区北京大学医学部基础医学院解剖/组织胚胎学系北京大学人民医院眼科

出处《中国科学：生命科学》 CSCD 北大核心 2013年第7期541-547,共7页 Scientia Sinica(Vitae)

基金国家重点基础研究发展计划(批准号:2009CB320900,2011CB510206和2011CB510200) 国家自然科学基金重点项目(批准号:30831160516)资助

关键词碘乙酸光感受器视网膜退行性变猫

分类号 R774.1 [医药卫生—眼科]

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中国科学：生命科学

2013年第7期

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对碘乙酸诱导猫视网膜光感受器退行性损伤的功能评估

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