肠道病毒71型IgM快速检测方法的比较

Comparison of methods for EV71 IgM detection

目的探讨胶体金免疫层析(GICA)及荧光-AlphalisA 2种快速检测肠道病毒71(EV71)IgM方法的灵敏度和准确性,为EV71-IgM临床检测方法选择提供依据。方法收集2010年3月到2012年8月在我院感染科住院的手足口病患者899例血清,分别用GICA、AlphalisA和ELISA法对EV71特异性IgM进行检测,计算各方法的总一致性和一致性百分比。结果 GICA法与AlphalisA法检测结果一致性很高,但2种方法与ELISA法的阴性率一致性很高,但其阳性率低于ELISA法。结论 ELISA法阳性率高于GICA法和AlphalisA法,但由于ELISA法操作较复杂且结果易受外界影响,故在基层医院可以对GICA和AlphalisA 2种方法根据临床需要来选用。

There have been several methods for entericvirus 71 IgM detection, however, among of which, each shows different accuracy and sensitivity. In this study we compared the characters of the routinely used methods to provide a suitable choice for clinical detection of EV71. We collected sera from 899 patients with hand-foot-and- mouth disease, who were admitted to our hospital during March 2010 to August 2012. The EV71 specific IgM was measured by gold immunochromatographic assay （GICA）, AlphalisA, and enzyme linked immunosorbent assay （ELISA） methods. Results showed that the sensitivity and accuracy of GICA were consistent with those of ELISA, however, the consistency for both methods was remarkably different from that for AlphalisA method. Results indicated that the accuracy for AlphalisA method is significantly higher than GICA and ELISA methods, however, considering the convenient and rapid manipulation of GICA and several affecting factors of ELISA, GICA and AlphalisA methods could be chosen for EV71 specific IgM detection in primary hospitals in China.