红外线光谱应用于牙周炎龈沟液分子标记物的分析

Periodontitis-specific Molecular Signatures in Gingival Crevicular Fluid by Infrared Spectroscopy

目的:评估红外线光谱检测龈沟液内牙周炎分子标志物的可行性。方法:获取牙周炎(n=129)、牙龈炎(n=125)和健康组织区(n=135)共389个区域的龈沟液样本的红外线光谱,使用多变量的统计学线性分析方法,对龈沟液吸收光谱的炎性指数定性评估。结果:通过红外光谱分析牙周炎组、牙龈炎组、健康组龈沟液中的4种分子标志物(酰胺酪氨酸I和酰胺酪氨酸II,DNA中的磷酸二酯基团的对称性的和非对称性的PO2-),可以准确地区分健康的和病变的牙周组织。LDA法推算红外线光谱在牙周炎诊断的准确率测试组为98.4%,验证组为93.2%。结论:红外线光谱可以应用于龈沟液中特定的炎性标志物的评估,有利于临床上对牙周组织炎症的诊断。

Objective： To evaluate whether infrared spectroscopy can be used to identify disease-specific molecular alterations to the overall biochemical profile of tissues and body fluids.Method： A total of 389 gingival crevicular fluid samples were obtained from periodontitis（n=129）,gingivitis（n=125） and normal sites（n=135）.Corresponding infrared absorption spectra of gingival crevicular fluid samples were acquired and processed,and the relative contributions of key functional groups in the infrared spectra were analysed.The qualitative assessment of clinical relevance of these gingival crevicular fluid spectra was interpreted with the multivariate statistical analysis-linear discriminant analysis.Results： Using infrared spectroscopy,we have been able to identify four molecular signatures（representing vibrations in amide I,amide II/tyrosine rings and symmetric and asymmetric PO2-stretching vibrations of phosphodiester groups in DNA） in the gingival crevicular fluid of subjects with periodontitis or gingivitis and healthy control subjects that clearly demarcate healthy and diseased periodontal tissues.Furthermore,the diagnostic accuracy for distinction between periodontally healthy and periodontitis sites revealed by multivariate classification of gingival crevicular fluid spectra was 98.4% for a training set of samples and 93.2% for a validation set.Conclusion： We have established that mid-infrared spectroscopy can be used to identify periodontitis-specific molecular signatures in gingival crevicular fluid and to confirm clinical diagnoses.