不同浓度低氧暴露对慢性疲劳综合征建模小鼠血清IL-6、TNF-α及IL-1β水平的影响

The Effect of Hypoxia Exposure on the Levels of Serum IL-6,TNF-α,and IL-1βin Mice with Chronic Fatigue Syndrome

目的:探讨不同浓度低氧暴露对慢性疲劳综合征(CFS)小鼠外周血促炎因子IL-6、TNF-α及IL-1β水平的影响。方法:雄性ICR小鼠72只,随机分为6组,每组12只,即建模正常对照组(C_0组)、慢性疲劳综合征建模组(CFS_0组)、低氧暴露正常对照组(C组)、低氧暴露慢性疲劳综合征对照组(CFS组)、慢性疲劳综合征+15%氧浓度低氧暴露组(15%H组)和慢性疲劳综合征+18%氧浓度低氧暴露组(18%H组),其中CFS_0组、CFS组、15%H组和18%H组采用束缚和强迫游泳方式建立慢性疲劳综合征小鼠模型,C_0组和C组同等条件下正常饲养。建模完成后C_0组和CFS_0组立即处死,检验建模效果;C组继续饲养7天,CFS组自然恢复7天,15%H组和18%H组分别在15%和18%氧浓度环境暴露7天(1小时/天),之后处死各组小鼠,检验低氧暴露效果。采用酶联免疫法检测各组小鼠外周血促炎因子IL-6、ITNF-α及IL-1β水平。结果:(1)CFS建模完成后,CFS_0组小鼠外周血促炎因子IL-6、TNF-α及IL-1β水平较C_0组显著升高(P<0.05)。(2)低氧干预后,15%H组外周血IL-6和IL-1β水平较CFS组呈下降趋势(P>0.05),IL-6较C组无显著性差异,但IL-1β仍显著高于C组(P<0.05);18%H组小鼠外周血IL-6和IL-1β水平与CFS组无显著性差异(P>0.05),仍明显高于C组(P<0.05);CFS组小鼠外周血IL-6和IL-1β水平仍显著高于C组(P<0.05),但各组间TNF-α水平无显著性差异(P>0.05)。结论:通过束缚和强迫游泳成功建立以外周血促炎因子IL-6、TNF-α和IL-1β升高为特征的慢性疲劳综合征小鼠模型,而在氧浓度为15%环境中暴露7天(1小时/天)有助于慢性疲劳综合征小鼠外周血促炎因子IL-6恢复到正常水平。

Objective To study the effect of hypoxia exposure on the levels of pro-inflammatory cytokine（IL-6,TNF-α,and IL-1β） in mice with chronic fatigue syndrome（CFS）. Methods 72 ICR male mice were randomly divide into 6 groups：normal group （C0）,CFS group （CFS0）,normal without hypoxia exposure group （C）, CFS without hypoxia exposure group （CFS）, CFS with 15%O2 hypoxia exposure group（15%H）,and CFS with 18%O2 hypoxia exposure group （18%H）. CFS of the mice was induced by restraint and forced swimming,and the normal mice were fed under the same conditions. Groups 15%H and 18%H received hypoxia exposure one hour per day for seven days. The blood samples were collected to detect serum IL-6,TNF-α,and IL-1β by enzyme. Results （1）Compared to group Co,plasma IL-6, TNF-α,and IL-1β levels in group CFS0 significantly increased after 4 weeks restraint and forced swimming stress. （2）Compared to group CFS,group 15%H exhibited a decreasing trend in IL-6 and IL- 1β levels,and there was no significant difference in the IL-6 level between groups 15%H and C ,but the IL-1βlevel was still higher than group C; No significant difference was observed in the IL-6 and IL-1β levels between groups 18%H and CFS,and the IL-6 and IL-1β levels in group 18%H were still higher than group C. No significant difference was found in TNF-α level among groups C ,CFS, 18%H and 15% H. Conclusion Four-week restraint and forced swimming stress successfully induces increase in pro- inflammation cytokines in mice with chronic fatigue syndrome. Moreover,hypoxia exposure one hour per day for seven days is beneficial for regaining pro-inflammation cytokines IL-6 to normal level.