高压氧对疲劳大鼠肾损伤的保护作用研究

Hyperbaric Oxygen Exposure Protects the Kidney Injury of Rats with Exercise-induced Fatigue

目的:探讨高压氧暴露对疲劳大鼠肾脏损伤的保护作用及机制。方法:4周龄健康雄性(Sprague IDawley,SD)大鼠30只,随机分为对照组、疲劳模型组和疲劳高压氧暴露组3组,每组10只。建立大鼠游泳致力竭疲劳模型并通过高压氧暴露进行恢复;8周后检测各组动物血清尿素氮(BUN)和肌酐(Cre);采用光镜观察肾脏组织形态结构,取0.5 g肾组织制备成10%肾组织匀浆,检测匀浆液丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性。结果:①疲劳高压氧暴露组大鼠游泳时间明显长于疲劳模型组(P<0,05)。②三组大鼠血清BUN和Cre含量存在显著性差异(P<0.01),两两比较,疲劳模型组BUN和Cre含量均显著高于其他两组(P<0.05)。③三组大鼠肾脏组织MDA含量存在显著性差异(P<0.01),两两比较,疲劳模型组MDA含量显著高于其他两组(P<0.01);三组肾脏组织SOD活性存在显著性差异(P<0.01),两两比较,疲劳模型组SOD活性显著低于其他两组(P<0.01)。④肾脏组织形态结构显示:对照组肾小管结构完整,基底膜保留完好,间质无充血现象。疲劳组肾小管结构紊乱,排列稀疏,肾小管和肾小球严重变性,细胞核固缩,髓质间质疏松。疲劳高压氧暴露组肾脏组织形态更接近对照组,虽然髓质间质疏松现象无明显改变,但肾小管和肾小球紊乱均有所改善。结论:高压氧暴露有效提高大鼠游泳时间,改善肾脏功能,降低疲劳大鼠肾脏MDA含量,提高SOD活性,改善肾脏组织病理变化,对疲劳大鼠肾脏损伤起到保护作用。

Objective To explore the protective effect of hyperbaric oxygen exposure on the kidney injury in rats with exercise-induced fatigue. Methods Thirty healthy 4-week old male SD rats were randomly divided into 3 groups： control,fatigue model and fatigue model plus hyperbaric oxygen exposure （HBO） groups. Rats in fatigue model group were treated with hyperbaric oxygen exposure. Blood urea nitrogen （BUN） and serum creatinine （Cre）,content of malondialdehyde （MDA） in kidney homogenates, and the pathological change in renal tissue of the rats were measured or observed 8 weeks after the experiment. Results （1） Swimming duration of the hyperbaric oxygen exposure group was significantly longer than that of the fatigue model group （P 〈 0.05）. （2）The content of BUN and Cre in renal tissue of rats revealed significant difference among the three groups （P 〈 0.01）,and in fatiguemodel group was significantly higher than that in other two groups （P 〈 0.05）. （3） The MDA content in renal tissue was significantly different among the three groups（P 〈 0.01 ）, and in fatigue model group was significantly higher than that in other two groups （P 〈 0.01）. The activity of SOD demonstrated significant difference among the three groups （P 〈 0.01）,and in fatigue model group was significantly lower than that in other two groups （P 〈 0.01 ）. serious degeneration of tubules and glomerulus （4） Disordered and sparsely disarranged renal tubules, ,karyopyknosis,rarefaction and porosity of medullary interstitial structure in renal tissue were seen in the fatigue model group,while the morphology of renal tissue in HBO group was more close to the normal control group. Conclusion Hyperbaric oxygen exposure effectively improves the swimming duration,reduces the content of MDA,increases the activity of SOD, alleviates the pathological changes of renal tissue in fatigued rats.