运用扫描电镜技术观察草莓白粉病菌

SEM studies on strawberry powdery-mildew

为探明草莓白粉病菌侵染过程中病原与植物之间的超微结构变化,并对草莓白粉病菌株的菌丝生长、菌落形态和孢子形成及形状有所了解,本文运用扫描电镜(SEM)观察了草莓白粉病菌在不同节位叶片上生长和侵染过程的超微形态。结果表明:在自然状态下,同一株新稍幼嫩、未见发病的叶片上也有病原菌的存在,且菌丝数量少,成熟叶片和老叶片表面均有菌丝和孢子生长附着,并且草莓白粉病菌丝在不同节位的叶片表面均随着叶片的生长过程而增加,病菌各阶段形态也随之丰富。

To verify and understand the key ultrastructure changes of the plant-fungus interaction during the infection process of the strawberry powdery mildew, scanning electron microscopy （SEM） was used to observe the growth and the infection process of the strawberry powdery mildew in different sections on the leaf. The results show that in natural condition, the pathogens with less mycelium also exist on the same new shoot. Mycelium and spores adhere to both the mature leaf and old blade surface. With the growth of the blade, the quantity of the mycelium is increased and the pathogen morphology on each stage is rich on different section of the plant.