结直肠癌患者血清IL-17和TGF-β浓度及其相关性的初步研究

Preliminary studies on the serum IL-17 and TGF-β levels and their correlations with tumor incidence and progression in colorectal cancer patients

目的:研究血清IL-17和TGF-β浓度变化与结直肠癌(colorectal cancer,CRC)发生发展的相关性及其临床意义。方法:健康志愿者30例、无远处转移并行手术治疗的结直肠癌患者59例及术后发生远处转移的44例患者被纳入本研究,依次为A(对照组)、B、C组,平均年龄分别为(53.8±20.8)岁、(62.0±11.8)岁、(64.0±15.7)岁,所有患者均经病理学检查确诊为结肠或直肠癌。B组59例,其中结肠癌26例为B1组,直肠癌33例为B2组;C组44例,其中结肠癌23例为C1组;直肠癌21例为C2组。测定A、B和C组血清IL-17和TGF-β浓度,其中分别测定B组术前术后血清IL-17和TGF-β浓度。结果:血清IL-17浓度测定结果显示,B组术前高于术后,C组高于B组(术前和术后),B、C组均高于A组,B组术前术后比较、B组和C组比较及B、C组与对照组比较差异均有统计学意义(P<0.05)。B组术前、术后血清TGF-β水平比较差异无统计学意义(P>0.05),A组和B组比较差异无统计学意义(P>0.05)。C组高于B组,B、C组均高于A组,C组和B组比较,B、C组和A组比较,差异均有统计学意义(P<0.05)。B组结肠癌(B1组)和直肠癌(B2组)组比较、C组结肠癌(C1)和直肠癌(C2)组比较,血清IL-17和TGF-β水平差异均无统计学意义(P>0.05)。结论:血清IL-17水平和结直肠癌的发生、发展密切相关,随着结直肠癌进展和肿瘤负荷增加,血清IL-17水平升高。血清TGF-β水平和结直肠癌转移显著相关,在结直肠癌的转移机制中可能起到重要作用。

Objective： This work aimed to investigate the relationships of the serum levels of IL-17 and TGF-β with the carcinogenesis and progression of colorectal cancer （CRC）, as well as the clinical significance of these serum levels. Methods： Data of 30 healthy subjects, 59 patients with simple CRC and 44 CRC patients with postoperative （post-op） metastasis were recruited in this study. The patients were respectively divided into group A （30 healthy subjects as the control group）, group B （59 CRC patients without distant metastasis after surgery）, and group C （44 CRC patients with post-op metastasis）. The patients in each group had a mean age of 53.8 ± 20.8, 62.0 ± 11.8, and 64.0 ± 15.7 years, respectively. All patients were confirmed by pathological diagnosis. The serum levels of IL-17 and TGF-β were measured by enzyme-linked immunosorbent assay. All quantitative data were analyzed using SPSS 13.0. Results： The IL-17 serum level was significantly higher in groups B and C than in group A. The preoperative （pre-op） serum level of IL-17 was significantly higher than the post-op serum level in group B （P〈0.05）. No significant difference was observed in the TGF-β serum levels between groups A and B, as well as between the pre-op and post-op serum levels in group B （P〉0.05）. However, the TGF-β serum level in group C was significantly higher than that in groups A and B （P〈0.05）. No significant correlation was observed in the serum level IL-17 or TGF-β between colon and rectum cancers in groups B and C. Conclusion： The serum level of IL-17 is significantly correlated with that of CRC. The serum level IL-17 increases with the aggravation of CRC and increased tumor burden. A strong correlation exists between the serum level of TGF-β and metastasis of CRC. Cytokine IL-17 and TGF-β may play an important role in the progression and metastasis of CRC.