人乳头瘤病毒E6/E7 mRNA检测联合宫颈活检在宫颈病变中的应用价值

Application value of human papilloma virus E6/E7 mRNA detection combined with cervical biopsy in diagnosis of cervical intraepithelial neoplasia

目的探讨经过新柏超薄液基细胞学检测(TCT)的标本检测高危人乳头瘤病毒(HPV)E6/E7 mRNA联合宫颈活检在宫颈病变筛查中的可行性及应用价值。方法采用杂交信号放大的核酸检测技术,对100例TCT示不同程度宫颈上皮瘤样病变和宫颈癌的标本进行高危HPV E6/E7 mRNA检测,并对HPV E6/E7 mRNA阳性者和阴性者同时行宫颈活检,同时就HPV E6/E7 mRNA表达在不同细胞学级别中的分布情况行统计学分析。结果不同细胞诊断级别E6/E7转录数有统计学差异(P<0.05),随着宫颈病变程度增加,HPV E6/E7 mRNA的转录数相应增加。HPV E6/E7mRNA的转录数越高,宫颈活检阳性率越高。结论高危HPV E6/E7 mRNA检测联合宫颈活检对宫颈上皮内瘤变和宫颈癌的筛查有临床意义。

Objective： To research the diagnosis value by determining high risk human papilloma virus（HPV） E6/E7 mRNA combined with cervical biopsy in the sample with cervical intraepithelial neoplasia after detected by liquid based thin-prep cytology test（TCT）. Methods： The high risk HPV E6/E7 mRNA was detected in 100 TCT samples with different degrees of cervical lesions. The cervical biopsy examination was done for all the patients with positive or negative of HPV E6/E7 mRNA test. The correlation between the expression of HPV E6/E7 mRNA and the cytological grades was analyzed. Results： The copy number of HPV E6/E7 mRNA was statistically different in various cytological grades （P〈0.05）. The copy number of HPV E6/E7 mRNA was correlated with severe degree of epithelial cervical dysplasia. The copy number of HPV E6/E7 mRNA was positively correlated with positive rate of cervical biopsy. Conclusions： High risk HPV E6/E7 mRNA test combined with cervical biopsy is of clinical value for diagnosis of cervical intraepithelial neoplasia.