血清与组织glactin-3联合检测在甲状腺癌早期诊断中的价值

Detection of glactin-3 in both serum and tissue for early diagnosis of thyroid carcinoma

目的探讨甲状腺癌患者血清与组织中半乳凝素-3(galectin-3,gal-3)的表达水平及意义。方法收集河北北方学院附属第一医院就诊甲状腺肿瘤患者159例,正常对照组16例。采用酶联免疫吸附法(ELISA)检测血清gal-3浓度,免疫组织化学SP和原位杂交技术法检测甲状腺手术标本中gal-3蛋白和mRNA的表达。结果 Gal-3蛋白和mRNA均表达于胞浆,呈棕黄色颗粒。66例乳头状癌gal-3表达阳性率96.97%;6例滤泡癌阳性率83.33%;36例乳头状增生阳性率16.67%;51例腺瘤gal-3阳性率11.74%;Gal-3mRNA阳性率略低于其蛋白,且与蛋白表达呈正相关。甲状腺癌组血清gal-3浓度明显高于良性乳头状增生组、腺瘤组和正常对照组(均为P<0.001)。血清gal-3浓度在甲状腺癌组织阳性组明显高于阴性组P<0.05。结论检测血清gal-3浓度及组织表达可以提高甲状腺癌的诊断率。

Objective To investigate the levels of galectin-3 in the serum and surgical specimens from patients with malignant and benign thyroid lesions and explore their clinical significance. Methods Serum samples were collected from 159 patients with thyroid neoplasms and 16 normal subjects for detection of galectin-3 level using ELISA. The expressions of galectin-3 protein and mRNA were also detected in the surgical specimens by immunohistochemistry and in situ hibridazation. Results Galectin-3 protein and mRNA were expressed at a rate of 96.67% in 66 cases of papillary thyroid carcinoma, 83.33% in 6 cases of follicular thyroid carcinoma, 16.67% in 36 cases of papillary hyperplasia, and 11.74% in 51 cases of thyroid adenoma. The expression level of galectin-3 mRNA was slightly lower than but positively correlated with its protein expression. Patients with thyroid carcinoma had significantly higher serum concentration of galectin-3 than those with benign thyroid lesions （papillary hyperplasia and thyroid adenoma） and normal subjects （P0.001）. In patients with papillary thyroid carcinoma, galectin- 3 positivity in the tumor tissue was associated with a significantly higher serum galectin-3 level in comparison with the negative cases （P0.05）. Conclusion Detection of galectin-3 in both the serum and surgical specimens can improve the diagnosis rate of thyroid carcinoma.