四种消毒方式对简易呼吸囊的灭菌效果评价

Sterilizing effect and residual bacteria of four different methods for sterilizing simple breathing vesicles

目的研究观察4种不同消毒方式对简易呼吸囊的消毒效果和微生物残留状况,探讨加强呼吸囊消毒方式方法管理的必要性。方法分4组消毒试验:G1组43例,500 mg/L二氧化氯(ClO2)喷雾;G2组28例,酒精喷雾;G3组47例,50 mg/L三氯异氰尿酸(TCCA)浸泡;G4组46例,50 mg/L ClO2溶液浸泡。各组于消毒后30 min取样接种培养,菌落计数后用卡方秩和进行统计学分析,并对残留菌鉴定分型。结果(1)4组菌落计数有显著性差异(P<0.001)。细菌残留率:G1组为0%,G2组53.6%,G3组27.7%,G4组21.7%,各组间比较统计学有显著性差异(P<0.001)。(2)残留菌鉴定结果显示铜绿假单胞杆菌和饱曼不动杆菌,溶血性葡萄球菌等常见耐药性致病菌。结论麻醉简易呼吸囊以500 mg/L ClO2喷雾方式消毒消果最好。中低效TCCA反复浸泡长期使用,可能导致耐药致病菌生长。

Objective To study the effects of 4 different methods for disinfection of simple breathing vesicles and microbial residue. Methods The disinfection tests were divided into 4 groups： G1 group （43 cases） with 500 mg/L chlorine dioxide spray, G2 group （28 cases） with alcohol spray, G3 group （47 cases） with 50 mg/L trichloro isocyanuric acid （TCCA） immersion, and G4 group （46 cases） with 50 mg/L chlorine dioxide solution immersion. Afrer 30 min of dsinfection, each group was examined by bacterial culture and colony count. The residual bacteria were identified and typed. Results The 4 methods showed significant differences in bacterial colony count （P〈0.001）. The rate of bacterial residue was 0% in G1 group, 53.6% in G2 group, 27.7% in G3 group, and 21.7% in G4 group, showing significant differences between the 4 groups （P〈0.001）. The residual bacteria included antibiotic- resistant common opportunistic pathogen such as Pseudomonas aeruginosa, Acinetobacter baumannii and Staphylococcus haemolytic. Conclusions Disinfection with 500 mg/L chlorine dioxide spray is the best for simple breathing vesicles. Prolonged immersion in TCCA may lead to the growth of drug-resistant pathogens in the breathing vesicles.