去势雄性大鼠阴茎海绵体CBS和CSE的表达变化

Expressions of cystathionine-β-synthase and cystathionine-γ-lyase in the corpus cavernosum smooth muscle of castrated rats

目的:研究胱硫醚-β-合成酶(CBS)和胱硫醚-γ-裂解酶(CSE)在去势大鼠阴茎海绵体平滑肌(CCSM)中的表达,探讨其在去势后勃起功能障碍发生中的作用。方法:60只8周龄雄性SD大鼠随机分成:假手术2周组(A组)、假手术4周组(B组)、去势2周组(C组)、去势4周组(D组)。术后检测各组血清睾酮(T)水平,免疫组化及RT-PCR检测CBS和CSE在大鼠CCSM中的表达。结果:血清T水平C组[(11.85±6.73)nmol/L]和D组[(1.96±1.23)nmol/L]分别较A组[(89.65±17.13)nmol/L]和B组[(106.75±19.68)nmol/L]显著下降(P<0.05)。CBS、CSE在各组大鼠CCSM中均有表达,CBS mRNA相对表达量C组(0.93±0.14)和D组(0.79±0.17)分别较A组(2.13±0.65)和B组(2.07±0.53)显著降低(P<0.05);CSE mRNA相对表达量C组(0.87±0.20)和D组(0.71±0.12)分别较A组(1.93±0.15)和B组(1.89±0.45)显著降低(P<0.05)。CBS蛋白积分光密度值(IA)C组(130.35±23.56)和D组(80.29±29.65)分别较A组(310.57±130.56)和B组(349.68±112.35)显著降低(P<0.05);CSE蛋白IA C组(93.56±36.64)和D组(58.56±19.95)分别较A组(269.56±116.76)和B组(298.35±100.76)显著降低(P<0.05)。T水平与CBS、CSE在CCSM中的表达水平具有正相关性。结论:T可能通过CBS、CSE的表达调控阴茎勃起功能。

Objective： To study the expressions of cystathionine-β-synthase （CBS） and cystathionine-γ-lyase （CSE） in the corpus cavernosum smooth muscle of castrated rats and their roles in erectile dysfunction after castration. Methods ： We randomly as- signed 40 eight-week-old male SD rats to groups A （2-week sham-operation）, B （4-week sham-operation）, C （2-week castration） and D （4-week castration）. We determined the level of serum testosterone （T） and the expressions of CBS and CSE in the corpus caverno- sum smooth muscle of the rats after operation using immunohistochemistry and RT-PCR. Results ： The T level was significantly de- creased in groups C （ [ 11.85 ± 6.73 ] nmol/L） and D （ [ 1.96 ± 1.23 ] nmol/L） as compared with A （ [ 89.65 ± 17.13 ] nmol/L） and B （[ 106.75 ± 19.68 ] nmol/L） （P 〈 0.05 ）. CBS and CSE were expressed in all groups of rats, but the relative expressions of CBS and CSE mRNA were significantly lower in groups C （0.93 ± 0.14 and 0.87 ± 0.20） and D （0.79 ± 0.17 and 0.71 ± 0.12） than in A （2.13 ± 0.65 and 1.93 ± 0.15） and B （2.07 ± 0.53 and 1.89 ± 0.45 ） （ P 〈 0.05）, so were the optical density values （IA） of the CBS and CSE proteins, 130.35 ± 23.56 and 93.56 ± 36.64 in group C and 80.29 ± 29.65 and 58.56 ± 19.95 in group D, as compared with 310.57 ±130.56 and 269.56 ± 116.76 in group A and 349.68 ±112.35 and 298.35 ±100.76 in group B （P 〈0.05）. The androgen level was positively correlated with the expressions of CBS and CSE in the corpus cavernosum smooth muscle of the rats. Con-clusion： Androgen regulates erectile function via the expressions of CBS and CSE.