血清贮存对前列腺特异性抗原检测结果的影响

Influence of serum storage on the laboratory results of prostate-specific antigen

目的:探讨血清贮存对检测血清T-PSA、F-PSA及F-PSA%(F-PSA/T-PSA)结果的影响。方法:应用全自动化学发光分析仪检测不同温度下贮存的血清F-PSA、T-PSA及F-PSA%值,并对各组数值结果进行比较分析。结果:①血清贮存在4℃和室温(22～26℃)时,F-PSA较T-PSA不稳定;室温下4 h后F-PSA值下降为(0.392±0.246)μg/L,较初始值比较有显著性变化(P<0.01),而T-PSA、F-PSA%分别下降为(1.522±1.085)μg/L和(25.03±5.94)%,无显著性变化(P>0.05)。室温下8 h,T-PSA、F-PSA分别降为(1.513±1.083)、(0.389±0.247)μg/L,与初始值比较均有显著性差异(P<0.05,P<0.01)。血清贮存在4℃,2 d后T-PSA、F-PSA及F-PSA%值分别降为(9.418±7.965)、(2.168±1.558)μg/L和(26.6±6.63)%,与初始值比较,均发生显著性变化(P均<0.05);贮存1周后三者结果分别为(9.203±7.736)μg/L、(2.047±1.478)μg/L和(25.64±6.56)%(P均<0.01)。血清贮存在-40℃时,4周后T-PSA、F-PSA及F-PSA%值分别为(4.532±4.393)、(1.178±1.034)μg/L和(24.45±8.81)%。②血清在-40℃反复冻融3次后检测T-PSA和F-PSA值分别为(5.982±5.314)μg/L和(1.341±1.029)μg/L,与初始值比较,无明显变化(P>0.05)。结论:血清贮存温度不同对血清T-PSA、F-PSA及F-PSA%的检测有不同程度的影响,且F-PSA较T-PSA变化大,F-PSA%相对稳定;血清贮存在-40℃,T-PSA和F-PSA至少可稳定1个月;血清反复冻融对PSA检测无影响。

Objective： To investigate the influence of serum storage on the laboratory results of serum T-PSA, F-PSA and F- Using automated chemiluminescence, we detected and compared the values of serum T-PSA, F-PSA and F-PSA% in the serum stored in different conditions. Results ： When the serum was stored at 4 ℃ or at the room temperature （22 - 26 ℃ ）, F- PSA was unstable as compared with T-PSA. Compared with the initial value, after 4 hours at the room temperature, F-PSA was de- creased to （0.392 ± 0.246）μg/L （ P 〈 0.01 ）, while T-PSA and F-PSA% to （ 1. 522± 1. 085 ） μg/L and （ 25.03± 5.94） %, re- spectively, with no significant difference; after 8 hours at the room temperature, T-PSA and F-PSA were reduced to （1. 513 ± 1. 083 ） and （0. 389 ± O. 247 ） μg/L （ P 〈 0.05 and P 〈 0. 01）. At 4 ℃, T-PSA, F-PSA and F-PSA% were decreased to （ 9.418 ± 7. 965 ） μg/L, （2. 168 ± 1. 558 ）μg/L and （ 26.6± 6.63 ） %, respectively, after 2 days （ P 〈 0.05 ）, and to （ 9. 203 ± 7. 736 ） μg/L, （ 2.047 ± 1. 478 ）μg,/L and （ 25.64 ± 6.56） % after 1 week （ P 〈 0.01 ）. At - 40 ℃, T-PSA, F-PSA and F-PSA% were （4. 532 ± 4. 393 ） μg/L, （ 1. 178 ± 1. 034） μg/L and （ 24.45 ± 8.81 ） % after 4 weeks. When the serum was stored at - 40 ℃ and after 3 freeze-thaws, F-PSA and T-PSA were （5. 982±5. 314） and （ 1. 341 ± 1. 029）μg/L, respectively, with no significant difference fromthe initial values. Conclusion： Different conditions of serum storage have different influences on the laboratory results of serum T- PSA, F-PSA and F-PSA%, more on F-PSA than on T-PSA, while F-PSA% is relatively stable. At -40 ℃, T-PSA and F-PSA may remain stable for a month at least. Repeated freeze-thaws of the serum do not affect the laboratory results of F-PSA and T-PSA.