依达拉奉联合乌司他丁对重症急性胰腺炎大鼠脑损伤的保护作用

The protective effects of edaravone combined with ulinastatin on the treatment of brain injury in rats With severe acute pancreatitis

目的研究依达拉奉联合乌司他丁对重症急性胰腺炎（SAP）大鼠脑损伤的保护作用。方法100只雄性远交系大鼠随机分为10组：正常对照6h组、48h组，SAP6h组、48h组，依达拉奉处理6h组、48h组，乌司他丁处理6h组、48h组，依达拉奉联合乌司他丁处理6h组、48h组；每组各10只。分别在术后第6h、48h观察神经功能评分。采集血液、胰腺组织、脑组织标本，观察血清淀粉酶水平、脑组织伊文蓝含量、脑组织含水量、胰腺组织及脑组织镜下病理学变化。应用放射免疫法检测血清肿瘤坏死因子（TNF）-α含量，逆转录-聚合酶链反应（RT—PCR）法检测脑组织TNF-α信使核糖核酸（mRNA）含量，免疫组织化学法检测脑组织核因子（NF）-κBp65活性。结果依达拉奉联合乌司他丁处理48h组血清淀粉酶水平、脑组织EB含量、脑组织含水量、胰腺组织及脑组织镜下病理学评分、血清TNF-α含量、脑组织TNF-αmRNA含量及脑组织NF-κBp65活性均较依达拉奉48h组、乌司他丁48h组及SAP48h组显著降低，而神经功能评分则显著升高（P〈0．01）。结论依达拉奉联合乌司他丁对SAP大鼠脑损伤有较好的保护作用。

Objective To investigate the protective effects of edaravone combined with ulinasta- tin on the treatment of brain injury in rats with severe acute pancreatitis. Methods A total of 100 male Sprague-Dawley rats were randomly divided into 10 groups that consisted of a normal control 6 and 48 hour group, a SAP 6 and 48 hour group, an edaravone treated 6 and 48 hour group, a ulinasta- tin treated 6 and 48 hour group, and finally a combination of edaravone and ulinastatin treated 6 and 48 hour group. Each group included 10 rats. Neurohehavioral scores were observed and a series of blood, pancreatic tissue, and brain tissue samples were obtained at 6 and 48 hours after operation. Serum am- ylase, brain tissue Evans Blue content, brain tissue water content, and the histopathological change of the pancreas and brain tissues were observed. Serum TNF-α was detected by RIA. The TNF-α mRNA and nuclear factor kappa B（NF-κB） p65 activity in brain tissues were measured with RT-PCR and im- munohistochemistry, respectively. Results In contrast to the 48 hour SAP, edaravone treated, and ulinastatin treated groups, the serum amylase, brain tissue Evans Blue content, brain tissue water content, histopathological score of the rat pancreas and brain tissue, serum TNF-α, TNF-α mRNA, and NF-KB p65 activity in brain tissues significantly decreased while neurobehavioral scores significant- ly increased in the 48 hour combined edaravone and ulinastatin treatment group （P〈0.01）. Conclusion Edaravone combined with ulinastatin can play a protective role on brain injury in rats with severe acute pancreatitis.