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MAGE mRNA和LUNX mRNA在非小细胞肺癌淋巴结的表达 被引量：2

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摘要 目的了解黑色素瘤抗原基因(MAGE mRNA)和肺特质性X蛋白基因(LUNX mRNA)在非小细胞肺癌(NSCLC)患者纵膈淋巴结中的阳性率。方法利用RT-PCR方法检测26例患者的123组淋巴结MAGE mRNA和LUNX mRNA的表达。结果 LUNX mRNA表达在NSCLC淋巴结微转移阳性率分别为42.3%(52/123)和34.1%(42/123),差异无显著性(P>0.05)。非MAGE肺癌患者淋巴结四种LUNX mRNA未见表达。常规病理检查和RT-PCR检测MAGE和LUNX mRNA在淋巴结微转移阳性表达率为30.1%(37/123)和44.7%(55/123),差异有显著性(P<0.05)。结论 MAGE mRNA是应用RT-PCR法检测NSCLC淋巴结微转移的较好的分子标志物,与LUNX mRNA联合检测可能有助于早期诊断肺癌淋巴结微转移。
作者 郭建极 刘涛 陈铭伍 周前 冼磊
机构地区 广西医科大学第一附属医院
出处 《中国老年学杂志》 CAS CSCD 北大核心 2013年第13期3048-3049,共2页 Chinese Journal of Gerontology
基金 广西自然科学资金(桂科自0991128 桂科青0728067)
关键词 非小细胞肺癌 黑色素抗原基因 肺特异性X蛋白基因 淋巴结 微转移
分类号 R734.2 [医药卫生—肿瘤]
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参考文献6

  • 1Massarelli E,Andre F, Liu DD, et al. A retrospective analysis of the out- come of patients who have received two prior chemotherapy regimens in- cluding platinum and docetaxel for recurrent non-small-cell lung cancer [J]. Lung Cancer,2003 ,39( 1 ) :55-61.
  • 2Iwao K, Watanabe T, Fujiwara Y, et al. Isolation of a novel human lung- specific gene, LUNX, A potential molecular marker for detection of mi- crometastasis in non-small-cell lung cancer [ J ]. Int J Cancer, 2001,91 (4) :433-7.
  • 3冼磊,王俊,姜冠潮,王丹蕾,刘军,李剑锋.MAGE基因在检测非小细胞肺癌淋巴结微转移中的应用[J].中国肺癌杂志,2005,8(3):219-222. 被引量：8
  • 4Jheon S, Hyun DS, Lee SC, et al. Lung cancer detection by a RT-nested PCR using MAGE A1-6 common primers [J]. Lung Cancer, 2004, 43 ( 1 ) :29-37.
  • 5姚舒洋,王潍博,崔言刚.LUNX、CK19和CEA基因在非小细胞肺癌淋巴结微转移中的比较研究[J].中华临床医师杂志（电子版）,2007,1(1):10-14. 被引量：3
  • 6Tajima K, Obata Y ,Tamaki H, et al. Expression of cancer/testis(ET) an- tigens in lung cancer[ J]. Lung Cancer,2003 ,42( 1 ) :25-55.

二级参考文献19

  • 1Ahrendt SA, Yang SC, Wu L, et al. Molecular assessment of lymph nodes in patients with resected stage Ⅰ non-small cell lung cancer: preliminary results of a prospective study. J Thorac Cardiovasc Surg,2002,123(3) : 466-474.
  • 2Tajima K, Obata Y, Tamaki H, et al. Expression of cancer/testis (CT) antigens in lung cancer. Lung Cancer,2003,42(1) : 23-33.
  • 3Fukagawa T, Sasako M, Mann GB, et al. Immunohistochemically detected micrometastases of the lymph nodes in patients with gastric carcinoma. Cancer,2001,92(4): 753-760.
  • 4Park JW, Kwon TK, Kim IH, et al. A new strategy for the diagnosis of MAGE-expressing cancers. J Immunol Methods,2002,266(1-2).
  • 5Mitas M, Cole DJ, Hoover L, et al. Real-time reverse transcription-PCR detects KS1/4 mRNA in mediastinal lymph nodes from patients with non-small cell lung cancer. Clin Chem,2003,49(2) :312-315.
  • 6Gu CD, Osaki T, Oyama T, et al. Detection of micrometastatic tumor cells in pN0 lymph nodes of patients with completely resec-ted nonsmall cell lung cancer: impact on recurrence and Survival.Ann Surg,2002,235(1): 133-139.
  • 7Su SL, Boynton AL, Holmes EH, et al. Detection of extraprostatic prostate cells utilizing reverse transcription-polymerase chain reaction. Semin Surg Oncol,2000,18(1) : 17-28.
  • 8Osaki T, Oyama T, Gu CD, et al. Prognostic impact of micrometastatic tumor cells in the lymph nodes and bone marrow of patients with completely resected stage Ⅰ non-small-cell lung cancer. J Clin Oncol,2002,20(13) : 2930-2936.
  • 9Hashimoto T, Kobayashi Y, Ishikawa Y, et al. Prognostic value of genetically diagnosed lymph node micrometastasis in non-small cell lung carcinoma cases. Cancer Res,2000,60(22): 6472-6478.
  • 10Wu J, Ohta Y, Minato H, et al. Nodal occult metastasis in patients with peripheral lung adenocarcinoma of 2.0 cm or less in diameter. Ann ThoracSurg,2001,71(6): 1772-1778.

共引文献9

同被引文献36

  • 1周芳,杜迎翔,周国华.基因表达量测定方法研究进展[J].药物生物技术,2004,11(4):267-270. 被引量：6
  • 2张俊旺,丁悌.Ⅰ期非小细胞肺癌淋巴结微转移的研究进展[J].肿瘤研究与临床,2004,16(5):355-358. 被引量：5
  • 3孙玉鹗.胸外科手术学[M].第2版.北京:人民军医出版社,2004.492-493.
  • 4Hung J J, Jeng WJ, Hsu WH, et al. Predictors, Of death, Local recurrence, And distant metastasis in completely re- sectedpathological stage - I non - small - cell lung cancer [J]. Thorac Oncol,2012; ,7(7) :1115-1123.
  • 5Jiang W, Chen X, Xi J, et al. Selective mediastinal lymph-adenectomy without intraoperative frozen section examinations for clinical stage I non- small- cell lung cancer: retrospective study of 403 cases [ J ]. orld Jsurg, 2013 ;37(2) :392-397.
  • 6Coello MC, Luketich JD, Litle VR, et al. Prognostic significance of micrometastasis in non- small- cell lung ancer[ J]. Clin Lung Cancer,2004 ;5(4) :214-215.
  • 7Kwiatkowski D J, Harpole DH, Strauss GM, et al. Molecular and pathologic markers in stage I non- small- cell zarcinoma of the lung [ J ]. J Clin Oncol, 1995 ; 13 ( 5 ) : [265-1279.
  • 8Allard WJ, Matera J, Miller MC, et al. Tumor cells circulate in the peripheral blood of all major carcinomas but not in healthy subjects or patients with nonmalignant diseases[J]. Clin Cancer Res ,2004 ;10(20) :6897-6904.
  • 9Chen JT, Cheng YW, Chou MC, et al. The correlation between aberrant connexin 43 mrna expression induced by promoter methylation and nodal small cell lung cancer [ J ]. Clin Cancer Res, 2003 ; 15 ; 9 ( 11 ) :4200-4204.
  • 10Moriya Y, Iyoda A, Kasai Y, et al. Prediction of lymph node metastasis by gene expression profiling in patients with primary resected lung cancer [ J ]. Lung Cancer, 2009;64( 1 ) :86-91.

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中国老年学杂志
2013年 第13期

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