载脂蛋白A5基因多态性对代谢综合征及其心血管事件发生的影响

The incidence of apolipoprotein AS gene polymorphism on the metabolic syndrome and cardiovascu- lar events DAI

目的探讨载脂蛋白A5（ApoA5）基因多态性对代谢综合征及其心血管事件发生的影响。方法选择代谢综合征患者200例[其中未合并心血管事件患者100例（MS-1组），合并心血管事件100例（MS-2组）]和健康人100例（对照组）。用ELISA法检测各组的载脂蛋白A5血清浓度，采用PCR—RFLP法测定三组的ApoA5—1131T〉C基因多态性。结果MS-1组ApoA5血清浓度明显低于对照组[（96．68±18．09）ng／mlVS（128．32±23．78）ng／ml，t=10．59，P〈0．01]；MS-2组ApoA5血清浓度亦低于MS-1组[（87．67±17．09）ng／mlVS（96．68±18．09）ng／ml，t=3．62，P〈0．01]；而且MS-2组、MS—1组的ApoA5—1131C基因型频率明显高于对照组（39．4％〉30％〉16．5％，P〈0．05）。结论代谢综合征及其心血管事件患者的ApoA5血清浓度明显降低，ApoA5—1131C与代谢综合征及其心血管事件相关。

Objective To study the effect of apolipoprotein A5 gene polymorphism on the metabolic syndrome and cardiovascular events. Methods A total of 200 patients with metabolic syndrome include 100 cases of unconsolidated cardiovascular events （ MS-1 group） and 100 cases of combined cardiovascular events （MS-2 group）; 100 cases of healthy people were used as control group. Apolipoprotein A5 serum concentration was detected by ELISA in each group. PCR-RFLP method was used to determine ApoAS- 1131T 〉 C gene polymorphism. The automatic analyzer was used to measure fasting plasma glucose （FPG）. Results The MS-l-group ApoA5 Serum concentration was significantly lower than the control group [ （96. 68 -＋ 18. 09）ng/ml vs （128.32 -＋ 23.78）ng/ml, t = 10. 59, P 〈 0. 01 ] ; the MS 2 groups ApoA5 se- rum concentrations was significantly lower than the MS-1 group [ （ 87.67 ＋ 17.09 ） ng/ml vs （ 96. 68 ＋ 18.09）ng/ml, t =3.62, P 〈0. 01 ] ; and ApoAS-1131C genotype frequency in MS-2 group and the MS-1 group was significantly higher than the control group （39.4% 〉30% 〉 16. 5%, P 〈0. 05）. Conclusions ApoA5 serum concentrations in patients with metabolic syndrome and cardiovascular events were significantly reduced, ApoAS-1131 C was related to the metabolic syndrome and cardiovascular events.