神经干细胞改善阿尔茨海默氏病转基因小鼠的学习和记忆能力

Neural stem cells improved learning and memory ability of Alzheimer's disease transgenic mice

目的本研究将绿色荧光蛋白(green fluorecent protein,GFP)转基因小鼠神经干细胞(neural stemcells,NSCs)移植到阿尔茨海默氏病(Alzheimer's disease,AD)转基因小鼠海马中,探讨NSCs在AD治疗中的作用。方法 E14-17d的GFP转基因小鼠的NSCs被分离、培养并诱导分化为神经细胞,相差显微镜和免疫细胞化学染色检测NSCs的表型与分化情况。通过显微注射,将GFP转基因小鼠的NSCs移植到AD转基因小鼠海马内,并以Morris水迷宫实验和旷场试验检测宿主鼠学习记忆能力的改善程度。结果培养的NSCs具有自我增殖能力,并可分化为神经元和星形胶质细胞。NSCs移植后,表达GFP的神经干细胞有效整合至宿主小鼠的海马组织内,并使宿主小鼠的学习和记忆能力明显改善。结论神经干细胞移植可改善阿尔茨海默氏病转基因小鼠的学习和记忆能力。

Objective In this study, neural stem cells（NSCs） from the green fluorescent protein（GFP） transgenic mouse were transplanted into the hippocampus of Alzheimer＇s disease （ AD ） mouse to explore the therapeutic efficacy of NSCs on AD. Methods NSCs of E14-17days were isolated, cultured and induced to be differentiated into neural cells. The phenotype and differentiation of NSCs were tested by phase contrast microscopy and immunocytochemical staining. NSCs from GFP transgenic mice were transplanted into the hippocampus of AD mice by micro-injection. The improvement of learning and memory ability was revealed by Morris water maze test and open field test. Results The isolated and cultured NSCs are capable of self-proliferating and differentiating into neurons and astrocytes. The learning and memory ability of AD mice was significantly improved by the transplantation of NSCs that integrated into host hippocampus. Conclusion The NSCs transplantation can improve learning and memory ability of AD transgenic mice.