不同穴位配伍电针刺对肝郁脾虚证大鼠结肠AQP4影响随机平行对照研究

The Random Control Experiment Research of the Effect of Different Specific Acupuncture Points on AQP4 of Liver Depression and Spleen Deficiency Rats

[目的]观察不同穴位配伍针刺对肝郁脾虚证大鼠结肠AQP4影响。[方法]使用随机平行对照方法,将60只大鼠按抽签方法随机分成6组(正常组、模型组、太冲三阴交组、太冲三阴交上巨虚组、太冲三阴交天枢组、太冲三阴交上巨虚天枢组)。实验第1d始对各组大鼠进行电针预处理(疏密波,频率80 Hz,强度1mA),1次/d,20min/次,连续7次,从上到下取双侧各组穴位,按照各组配穴的不同进行针刺,以局部皮肤肌肉轻微颤动为度。第8～22d,对除A组外的各组大鼠使用慢性应激方法复制肝郁脾虚证模型,随机选择(束缚、断食、断水、黑白颠倒、温水游泳)方法,每种方法实施3次,共造模15d。第23d,所有大鼠禁食24 h,称重,乙醚麻醉解剖后取结肠组织。观测大鼠外观行为、结肠组织AQP4。[结果]外观行为变化A组未见明显异常,与A组相比,造模第7d时,B组表现出活跃程度减弱、饮食减少、易醒、大便质地变软;C、D、E、F组未见明显改变;造模15d后,B组情绪低落,表情淡漠,争斗减少,饮食减少,大便转为稀溏,排便次数增多,毛发枯乱发黄,活动渐少,喜倦卧等;C、D、E、F组大鼠未见明显改变。结肠组织AQP4 F组灰度值与B组相比明显增大(P<0.01),阳性表达最弱;E组灰度值与B组相比也具有较高优势(P<0.01);C组与D组的AQP4灰度值与B组的相比均有一定升高(P<0.05),但弱于E组与F组;A组与B组相比AQP4灰度值大于B组(P<0.05)。[结论]不同穴位配伍针刺肝郁脾虚证大鼠能良性调节结肠AQP4含量,太冲三阴交上巨虚天枢配伍效果突出。

To observe the effect of different specific acupuncture points on AQP4 of liver depression and spleen deficiency rats.[Methods]To use random parallel control method,60 wistar rats were divided into 6 groups in random by the draw,which were normal control group,model control group,taichong sanyinjiao group,taichong sanyinjiao shangjuxu group,taichong sanyinjiao tianshu group,taichong sanyinjiao shangjuxu tianshu group.From the first day,every group requires electric acupuncture preconditioning in rats（dilatational wave,the frequency of 80Hz,intensity of 1mA）,one time a day,20 minutes a time,seven consecutive,taking each point on both sides from top to bottom.Carried out in accordance with the equipping hole different acupuncture,until the local skin muscle slight tremor.From 8th to 22nd day,each group of rats chronic stress method is used to copy the liver spleen deficiency model in addition to Group A outside,selecting methods in random（bondage,break feed,break water,reverse day and night,warm water swimming）.Every method is carried out three times,made the model for 15 days.All the rats stop feeding 24 hours from 23rd day,weighing and anesthesia by ether,getting colon tissue after dissection.Observe the appearance of the behavior of rats and AQP4.[Result]The appearance of the behavior,Group A did not see any abnormalities.On 7th day,Group B showed the activity more decreeased,eating less,wake up more easier,stool soft texture than Group A.Group C,D,E,F did not see any abnormalities.After 15 days,Group B began to low mood,expression indifferent,the struggle to reduce,eating less,dilute stool pond,defecate number increase,hair became yellow,activity more decreased,liked lying and so on.Group C,D,E,F did not see any abnormalities.The grey value of Group F of AQP4 increase more significant than Group B（P0.01）,the weakest positive expression.The grey value of Group E has increased significantly than Group B（P0.01）.The grey value of Group C and D have a modest increase than Group B（P0.05）,but weaker than Group E and F.The grey value of Group A is hihger than Group B（P0.05）.[Conclusion] Different acupoints compatibility needle liver spleen deficiency can regulate the colon benign AQP4 content in rats,then the taichong sanyinjiao shangjuxu tianshu group has the significant effect.