不同产地当归中阿魏酸的含量分析

Determination of ferulic acid in Angelica sinensis produced in different places

目的:测定不同产地当归中阿魏酸的含量,探讨产地对当归质量的影响。方法:采用高效液相色谱法(HPLC)对不同产地当归中阿魏酸含量进行测定,以乙腈-0.085%磷酸溶液(17∶83)(V/V)为流动相,以Agilent Extend-C18柱(4.6mm×250mm,5μm)为固定相,流速为1.0mL/min,柱温为35℃,检测波长为316nm。结果:阿魏酸在24.260～242.600ng范围内呈良好线性关系(r=0.99996),平均回收率为98.61%,RSD为1.74%。结论:各产地当归中阿魏酸含量存在差异,以甘肃岷县的当归阿魏酸含量为最高。本实验为当归的道地性提供了科学依据。

To determine the content of ferulic acid in Angelica sinensis produced in different places and to investigate the effect of place of origin on the quality of Angelica sinensis.Methods：High-performance liquid chromatography（HPLC） was used to determine the content of ferulic acid in Angelica sinensis produced in different places.HPLC was performed on Agilent Extend C18 column（4.6mm × 250mm,5μm） with a mobile phase of acetonitrile-0.085% phosphoric acid solution（V/V 17∶ 83） at a flow rate of 1.0 ml/min,a column temperature of 35℃,and a detection wavelength of 316 nm.Results：Ferulic acid showed a good linear relationship between concentration and peak area in the range of 24.260-242.600 ng（r = 0.99996）.The average recovery rate was 98.61%,and the relative standard deviation was 1.74%.Conclusion：Angelica sinensis produced in different places shows differences in ferulic acid content.Angelica sinensis produced in Min County,Gansu Province,China has the highest ferulic acid content.This experiment provides a scientific basis for the quality evaluation of Angelica sinensis.