尺胫针法对甲醛致小鼠足肿胀及血清PGE_2的影响

The Effect of Ulnar Tibial Acupuncture on Foot Swelling Mice Induced by Formaldehyde and Its Serum PGE_2

目的观察尺胫针疗法对小鼠足部急性炎症肿胀度及血清PGE2含量的影响。方法于小鼠右后肢足底第2、3跖趾关节间皮下注射2.5%甲醛液诱导急性炎症模型,分别给予尺胫针法和腕踝针法干预。分别采用游标卡尺法和双抗体两步夹心酶联免疫吸附法(ELISA)检测足跖肿胀度和血清PGE2含量。结果两种针法均可有效抑制甲醛致炎小鼠足跖肿胀度,以第二次治疗后为著,其作用尺胫针组优于腕踝针组。结论尺胫针法对甲醛致炎小鼠急性炎症有较好地抗炎作用,并通过降低血清中PGE2含量从而控制炎症的发展。

Objective To observe the effect of ulnar tibial acupuncture on the content of serum PGE2 and foot swelling of acute inflammation in mice. Methods gave the injection of 2.5 % formaldehyde on the right second and third hind foot of mice, solution to induce acute inflammation model, given the scale of ulnar tibial acupuncture and the wrist and ankle needling intervention respectively. Using vernier caliper method and double antibody two step sandwich enzyme - linked immunosorbent assay （ELISA） detection paw swelling degree and the content of PGE2 in serum. Results the two kinds of method could effectively inhibit the formaldehyde inflammatory mouse paw swelling, after second times treatment, the effect of ulnar tibial needle group was better than that of wrist - ankle acupuncture group. Conclusion ulnar tibial method has better anti - inflammatory effect on acute inflammation induced by formaldehyde in mice, and by reducing the develop- ment of inflammation and controlling the content of PGE2 in serum.