小鼠组蛋白去乙酰化酶基因序列分析

Sequence Analysis of Histone Deacetylase Genes in Mouse

利用生物信息学方法,通过NCBI和其他生物学数据库及DNAstar、Clustal X等生物信息学软件对小鼠11种去乙酰化酶(histone deacetylase,HDAC)的基因结构、开放阅读框、GC含量、氨基酸序列、同源性及染色体定位等问题进行了分析。结果发现小鼠HDACs外显子从10～29个不等,开放阅读框长度从1044～3450bp不等,GC含量约为50%。序列比对分析后发现HDAC1和HDAC2蛋白质序列之间相似性达89.8%,其余HDACs蛋白质序列之间相似性相对较低,HDAC7和HDAC8之间仅为8.8%。系统发生分析表明小鼠11种HDACs也按照酵母种系发育中不同HDACs的结构聚类为Ⅰ、Ⅱa、Ⅱb和Ⅳ等4个类群,来源于基因复制。染色体定位分析发现除HDAC6和HDAC8位于X染色体外,其余均位于常染色体。研究结果为进一步研究小鼠HDACs转录调控的分子机制和蛋白质功能奠定基础。

Based on NCBI and other bioinformatics database and software such as DNAstar and Clustal X, the gene struc- ture, GC contents, open reading frame, amino acid sequence homology and chromosomal localization of 11 histone deacety- lase （HDAC） genes of murine were analyzed. The results showed that the numbers of murine HDACs exons were between 10 and 29, the lengths were between 1044 bp and 3450 bp, and the GC contents were about 50%. Protein sequences analysis showed that HDAC 1 was highly homologous to HADC 2 （89.8%）, however, HDAC 7 and HDAC 8 had only 8.8% ho- mology. Furthermore, the 11 murine HDACs were separated into 4 different clades by the phylogenetic tree. Chromosomal localization analysis indicated that HDAC 6 and HDAC 8 were located in chromosome X, while the others were in euchromo- some. In conclusion, these results revealed the basic biological characteristics of murine HDACs, and provided basic data for the research on the molecular mechanism and protein function of transcriptional regulation.