摘要
目的:探讨葡萄籽原花青素对脑缺血再灌注(I/R)大鼠的转化生长因子β1(TGF-β1)表达的影响。方法:采用改进的Longa方法制作大鼠脑缺血再灌注模型,动物分为正常对照组、假手术组、脑缺血再灌注组和药物干预组。药物干预组在手术前1h给予葡萄籽原花青素,之后每天同一时间给药,直到被处死。药物干预组和缺血再灌注组于术后以及处死前分别两次给予神经功能评分。大鼠处死并取出样本分别进行HE染色和TGF-β1免疫组化染色。结果:大鼠神经功能评分显示大鼠造模成功率约为88%,TGF-β1在正常SD大鼠海马区神经元细胞和神经胶质细胞中有少量表达,缺血再灌注组各亚组阳性表达较正常对照组均明显增高(P<0.05);药物干预组各亚组与正常对照组比较TGF-β1表达均升高(P<0.05),在相同时间点TGF-β1表达趋势基本与I/R组相同,但阳性表达明显高于I/R组(P<0.05)。结论:葡萄籽原花青素能通过增加TGF-β1表达,以达到脑缺血再灌注的保护作用。
To investigate the effects of grape seed proanthocyanidin（GSP） on the expression of TGF-β1 in SD rats with brain I/R injury.Method;The middle cerebral artery ischemic reperfusion rat model was made using the improved Longa＇s method. The SD rats（250g-320g） were randomly divided into four groups,the normal control group（n = 10）,sham-operated group（n = 10）,ischemia-reperfusion group（I/R group,n = 20） and GSP group（the treatment group,n = 20）,The treatment group was given GSP at Ih before the operation and continuously given GSP at the same time evenyday till sacrificed by decapitation.The neurological function score of the SD rats in I/R group and the treatment group were recorded before and after the sacrifice.The samples were stained with HE and TGF-β1 immunohistochemical method.Result：The neurological function score showed the model success rate was 88%.Low TGF-β1 expression was shown mainly in neurons and neuroglial cells in hippocampus in the normal rats,and that was upregulated in I/R group（P0.05）.Compared with that of the normal controls,the expression of TGF-β1 in the treatment group was increased significantly（P 0.05）.The expression of TGF-β1 in the treatment group and the normal group was similar at the same time.Conclusion：GSP exhibits neuroprotective effects through promoting the expression of TGF-β1.
出处
《中国药师》
CAS
2013年第7期935-937,共3页
China Pharmacist
基金
湖南省科技厅课题(编号:2011TT2006)
