不同种、不同产地黄芪中黄酮类HPLC指纹图谱研究

HPLC Fingerprints of Flavonoid in Radix Astragali from Various Habitats

目的:对不同种、不同产地的黄芪药材中黄酮类成分建立HPLC指纹图谱。方法:采用HPLC法,乙腈·水二元梯度洗脱;流速0.8 ml·min^(-1);检测波长260 nm;柱温:35℃结果:10批黄芪药材有16个共有峰,其HPLC指纹图谱基本一致,各共有峰保留时间的比值基本一致,共有峰面积之和均大于总峰面积的90%,但其共有峰面积的比值有一定差异。结论:该方法重现性好,建立的指纹图谱为黄芪的质量控制及评价提供了依据。

To establish an HPLC fingerprints analysis method for flavonoid in Radix Astragali from various habitats. Method：The flavonoid was determined by HPLC.The mobile phase consisted of acetonitrile and water at a flow rate of 0.8 ml·min^（-1） with gradient elution.The UV detection wavelength was set at 260 nm and the column temperature was 35℃.Result;There were 16 common peaks in the chromatogram of each sample.The profile of HPLC fingerprints and the ratios of the common peak area for 10 samples were approximately the same.The sum of the common peak area was more than 90%of the total peak area of each sample,while there were some differences in the ratios of the common peak area among the samples.Conclusion：The method shows promising repeatability and reproducibility.The fingerprints can be used as a quality control method for Radix Astragali.