摘要
目的:改进飞龙掌血药材含量测定方法。方法:采用高效液相色谱法,色谱柱为Thermo ODS-2HYPERSIL柱(250mm×4.6 mm,5μm),以甲醇-水为流动相,梯度洗脱,检测波长283 nm,流速为1.0 ml·min^(-1)。结果:白屈菜红碱的线性范围为0.444~1.554μg(r=0.999 8),平均回收率为97.89%,RSD为0.96%(n=6)。结论:该方法简便、准确、重复性好,可用于飞龙掌血药材的质量控制。
To improve the method for the determination of chelerythrine in Toddalia Asiatica.Method:The separation was performed on a Thermo ODS-2HYPERSIL(250 mm×4.6 mm,5μm) column with the detection wavelength of 283 nm.The mobile phase was methanol-water with gradient elution at a flow rate of 1.0 ml·min^(-1).Result:The linear range of chelerythrine was 0.444-1.554μg(r =0.999 8),the average recovery was 97.89%(RSD =0.96%,n =6).Conclusion:The method is accurate, simple and reproducible,and can be used to control the quality of Toddalia Asiatica.
出处
《中国药师》
CAS
2013年第7期998-999,共2页
China Pharmacist
基金
济南军区后勤科研计划(编号:CJN10L067)
关键词
飞龙掌血
白屈菜红碱
含量测定
Toddlia asiatica(L.)Lam
Chelerythrine
Determination