急性肺损伤大鼠肺组织TNF-α和IL-1β的表达及塞来昔布对其影响

Effect of celecoxib on TNF - α and IL - 1β in lung tissue of rats with acute lung injury

目的初步探讨塞来昔布对急性肺损伤大鼠肺组织TNF-α和IL-1β表达的影响。方法将60只大鼠随机分为正常对照组、模型组、治疗组、药物对照组,每组15只,模型组和治疗组采用尾静脉注射LPS(5 mg/kg)建立急性肺损伤模型,后者在模型建立30 min后予塞来昔布灌胃,正常对照组和药物对照组在相同时间点分别予生理盐水和塞来昔布灌胃。采用逆转录-聚合酶链反应(RT-PCR)和蛋白质印迹(western blot)方法分别检测4组大鼠肺组织COX-2蛋白及TNF-α、IL-1βmRNA和蛋白的表达。结果模型组和正常对照组COX-2蛋白及TNF-α、IL-1βmRNA和蛋白表达比较均有显著性差异(P均<0.05);治疗组TNF-α、IL-1βmRNA和蛋白表达明显降低,与模型组均有显著性差异(P均<0.05)。结论选择性COX-2抑制剂塞来昔布可使急性肺损伤大鼠炎症反应减轻。

Abstract ： Objective It is to study the effect of celecoxib on the expression of TNF-α and IL - 1 β in lung tissue in acute lung injury rats. Methods Sixty rats were randomly divided into four groups： normal control group, model group, treatment group and drug control group, each group had 15 rats. Acute lung injury rat models were established by LPS induction（5 mg/ kg） in the tail vein in model group and treatment group, and the latter group was given celecoxib by intragastricadministration in 30min after model establishing; normal control group and drug control group were given equal saline water and celecoxib re- spectively at the same time. The expressions of TNF-α and IL - 1 βmRNA were evaluated by reverse transcription polymerase chain reaction（ RT - PCR） ; the expressions of COX - 2, TNF -α and IL - 1 β protein were detected by western blot analysis. Results Compared with normal control group, COX - 2, TNF -α and IL - 1 β protein and TNF -α and IL - 1 β mRNA expression in model group were significantly different （ P 〈 0.05） ; TNF -α and IL - 1 β mRNA and protein expressions in treatment group were significantly decreased, and compared with model group, they have statistical significance（P 〈 0.05）. Conclusion Celecoxib can inhibit inflammation reaction in acute lung injury rats.