喉癌患者与健康人血浆蛋白质差异表达谱的建立及质谱分析

Screening and mass spectrometry analysis of differentially expressed proteins of plasm between laryngocarcinoma and healthy individuals

目的:分析喉癌患者与健康人血浆中差异表达的蛋白质组,筛选潜在的与喉癌患者诊断密切相关的血浆分子标志物。方法:取喉癌患者与健康人空腹血浆标本各6例,分别等量混合成2组样本,利用双向电泳(2-DE)及基质辅助激光解吸电离飞行时间质谱(MALDI-TOF/TOF)技术筛选及鉴定差异表达的蛋白点,并对其进行生物信息学分析。结果:喉癌患者与健康人血浆相比较,筛选出表达量差异1.5倍以上的差异蛋白点共20个,其中表达上调的有8个,表达下调的有12个,进行质谱分析后,表达上调的蛋白有α-1抗胰蛋白酶、α-1酸性糖蛋白1、载脂蛋白L1、C反应蛋白、结合珠蛋白、血浆铜蓝蛋白;表达下调的蛋白有转铁蛋白、α-2-HS-糖蛋白、纤维蛋白原γ链、结合珠蛋白相关蛋白、免疫球蛋白λ-1链C结构域、免疫球蛋白κ链C结构域、载脂蛋白A1、转甲状腺素蛋白、载脂蛋白C3。结论:喉癌患者与健康人血浆的蛋白质表达谱表现出一定差异,这些差异蛋白有可能成为喉癌患者诊断的特异性血浆肿瘤标志物。

Objective：To compare and analyze the expression difference of proteins between laryngocarcinoma and healthy individuals to search for protein biomarkers that may be detected in plasm of laryngocarcinoma patients.Method：Pooled plasm from 6 laryngocarcinoma patients and 6 healthy individuals as controls were collected.Two-dimensional gel electrophoresis（2-DE） was used to isolate the total proteins,and the differential protein spots were identified by MALDI-TOF/TOF mass spectrometer,and then the biological information of the proteins was analyzed.Result：Twenty differential expressed protein spots with more than 1.5-fold between the laryngocarcinoma and healthy individuals were selected,and there were 8 proteins upregulated and 12 proteins downregulated among these proteins.After identifing by MALDI-TOF/TOF,compared with healthy controls,the spots that were upregulated in laryngocarcinoma patients were： alpha-1-antitrypsin,alpha-1-acid glycoprotein 1,apolipoprotein L1,C-reactive protein,haptoglobin,ceruloplasmin;the spots that were downregulated were： Serotransferrin,alpha-2-HS-glycoprotein,fibrinogen gamma chain,haptoglobin related protein,Ig lambda-1 chain C regions,Ig kappa chain C region,apolipoprotein A-I,transthyretin,apolipoprotein C-Ⅲ.Conclusion：Compared with laryngocarcinoma and healthy individuals,the plasm proteins profile showed differently.The proteins of differential expressed are expected to be the specific plasm biomarkers for laryngocarcinoma patients.