萝卜UV-B受体基因UVR8的电子克隆及序列分析

In silico Cloning and Sequence Analysis on UV - B Receptor Gene of UVR8 in Raphanus sativus

目的:电子克隆获得UV-B受体UVR8基因编码序列。方法:利用已知的芜菁UV-B受体基因UVR8基因序列,采用电子克隆的方法获得了萝卜UVR8基因cDNA的序列。对其编码蛋白序列进行了预测,并将其编码蛋白同芜菁和拟南芥的UVR8蛋白进行了同源性比较,对其三维结构进行了同源模建。结果:萝卜UVR8基因编码435个氨基酸。其同拟南芥UVR8蛋白的同源性高于芜菁。其三维结构显示其是两个单体形成二聚体发挥生理功能。结论:初步获得萝卜WUS基因的编码序列,为进一步研究其功能打下基础。

Objectlve：Obtain the sequence ceding UVR8 gene in Raphanus sativus. Method：With UVR8 gene in Brassica rapa, the cDNA of UVR8 gene, the receptor of UV - B in Raphanus sativus, was in silico cloned. Amino acid sequence ceded by UVR8 of Raphanus sativus was predicted and homology compared with Arabidopsis thaliana and Brassica rapa. Its three - dimensional structure was homology modeled. Result： The results showed that t UVR8 of Raphanus sativus ceded a protein of 435 amino acids. The homology of UVR8 protein between Raphanus sativus and Arabidopsis thaliana was higher than that between Raphanus sativus and Brassica rapa. Three -dimensionalstructure showed that two monomers combined to a dimer when its physiological function played. Conclusion：The UVR8 gene coding se- quence of Raphanus sativus was cloned preliminary, and laid the foundation for further study its function.