口腔鳞状细胞癌中eIF4E表达的临床意义及预后的相关性分析

Clinical significance of eIF4E expression and its correlation with prognosis of oral squamous cell carcinoma

目的:探讨真核细胞翻译起始因子4E(eukaryotic initiation factor 4E,eIF4E)在口腔鳞状细胞癌(OSCC)中的表达及临床意义。方法:采用RT-PCR、Western blot方法及免疫组化SP法检测30例OSCC新鲜组织及112例石蜡包埋组织与30例癌旁正常黏膜组织中eIF4E的表达,分析eIF4E表达与临床病理特征及预后的关系。结果:RT-PCR结果表明eIF4E mRNA在OSCC组织中的表达高于癌旁正常口腔黏膜(P<0.05);Western-blot结果显示eIF4E蛋白在OSCC中高于正常黏膜(P<0.05);多重比较显示,低分化和淋巴结转移病例中eIF4E的表达较高(P<0.05)。Log-Rank生存分析显示eIF4E蛋白低表达组和高表达组的5年生存率分别为63.9%和41.0%(P<0.05);COX多因素分析结果显示,eIF4E蛋白高表达是影响OSCC患者预后的指标之一。结论:eIF4E的高表达与OSCC的发生发展及不良预后密切相关。

Objective：To investigate the expression of eIF4E and its correlation with the clinicopathological parameters and prognosis of oral squamous cell carcinoma（OSCC）.Methods： eIF4E mRNA and protein expression in 30 cases of fresh OSCC samples and normal oral mucosa were detected by reverse transcriptase polymerase chain reaction（RT-PCR） and Western-blot respectively.eIF4E protein expression was examined by immunohistochemical（IHC） SP method in 112 cases of paraffin imbedded OSCC samples and 30 of OSCC adjacent tissue.Results： eIF4E mRNA and protein expression in OSCC was higher than in adjacent oral mucosa（P〈0.05）,and was related to the pathological differentiation and lymph node metastasis.Log-Rank analysis showed that 5-year survival rate of patients with high and low expression of eIF4E was 41.0% and 63.9% respectively（P〈0.05）.The Cox model analysis showed that eIF4E protein expression was related to the poor prognosis of OSCC.Conclusion： eIF4E overexpression is closely related with the occurrence and development of OSCC.