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超量表达BoFLC3、AtFT对芥菜开花期和抗寒性的影响 被引量:4

Influence of Over-expression of the BoFLC3 and AtFT Genes on Flowering- time and Cold Tolerance in Brassica juncea
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摘要 将来自甘蓝的BoFLC3基因和拟南芥的AtFT基因在芥菜中单独或共同表达发现,BoFLC3超量表达后,无论是长日照还是短日照条件下,转基因芥菜植株开花时间均明显延后;BoFLC3超量表达植株低温春化处理后,相对于未春化处理的转基因植株出现花期提前现象,但仍晚于非转基因对照植株;AtFT基因超量表达植株开花时间比非转基因对照植株大幅度提前。BoFLC3植株与AtFT植株杂交获得的BoFLC3和AtFT共表达植株开花时间与非转基因对照基本接近,显示BoFLC3和AtFT基因超表达对植物开花时间的影响效应可以相互抵消。同时,超量表达BoFLC3基因的芥菜植株抗寒性大幅度提高,而AtFT基因的超量表达不影响植株抗寒性,表明BoFLC3基因可能参与了植物的抗寒反应。 BoFLC3 gene from Brassica oleracea and AtFT gene from Arabidopsis were overexpressed alone or together in Brassica juncea. The results showed constitutive expression of the BoFLC3 gene in Brassica juncea significantly delayed flowering under long-day photoperiods as well as short-day photoperiods. Vernalization treatment induced earlier flowering of BoFLC3 overexpression plants than unvernalized transgenic control but still were flowering later than the wild type plants; the overexpression of the AtFT gene could induce early flowering in Brassica juncea. The flowering time of BoFLC3 and AtFT genes co-expression plants after cross were almost at the same time compared with the wild type plants,indicated the effects of the over-expression of the BoFLC3 and AtFT genes on flowering time could cancel each other out. Moreover,the cold-tolerance was enhanced by constitutive expression of the BoFLC3 gene in Brassica juncea,but no cold-tolerance changes were observed in AtFT overexpression plants. The results indicated the BoFLC3 should involve in plant cold-tolerance regulation.
出处 《园艺学报》 CAS CSCD 北大核心 2013年第7期1278-1288,共11页 Acta Horticulturae Sinica
基金 重庆市自然科学基金项目(cstc2011jjA80014) 中央高校基本科研业务费项目(XDJK2009B024) 国家科技支撑计划项目(2012BAD02B01-4) 重庆市科技攻关项目[CSTC2012GG(8005)]
关键词 芥菜 BoFLC3基因 AtFT基因 开花时间 抗寒性 Brassica juncea BoFLC3 gene AtFT gene flowering time cold-tolerance
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