miR-34a对鼻咽癌细胞增殖的负性调控作用

Inhibitory Effect of MiR-34a on the Proliferation of Nasopharyngeal Cancer Cells

目的:研究miR-34a对鼻咽癌细胞的增殖能力的影响。方法:使用PCR检测正常鼻咽上皮细胞及鼻咽癌细胞中miR-34a的表达水平,瞬时转染后通过MTT、克隆形成实验及凋亡实验探讨miR-34a对鼻咽癌细胞生物学行为的影响。结果:miR-34a在鼻咽癌细胞中表达量低于正常细胞,miR-34a抑制鼻咽癌细胞增殖及克隆形成能力,诱导凋亡。

Objective： To investigate the effect of miR-34a on the growth of nasopharyngeal cancer ceils in vitro. Methods： The expression of miR-34a both in normal nasopharyngeal epithelium and na- sopharyngeal cancer cell lines were assayed by quantitative PCR. MTT assay, clone formation as- say and flow cytometry were also applied to study the effect of miR-34a on nasopharyngeal cancer cells. Results： miR-34a expression was decreased in nasopharyngeal cancer cell than in normal na- sopharyngeal epithelial cell. miR-34a up-regulation contributed to a dramatically reduction of pro- liferation and clone formation while it also showed an increasing induction of apoptosis in CNE1 and CNE2 cell lines. Conclusion： miR-34a expression was downregulated in nasopharyngeal canc- er cells and its overexpression in vitro resulted in a greatly reduction of cell proliferation as well as a promotion of apoptosis in nasopharyngeal cancer cells.