美丽硬仆骨舌鱼核型分析

Karyotype Analysis of Scleropages formosus

美丽硬仆骨舌鱼(Scleropages formosus)是一种古老的具有很高经济价值的观赏鱼类。为了解该鱼的细胞遗传背景,采用胸腔注射植物血球凝集素(PHA)和秋水仙素的方法,以头肾为材料,空气干燥法制片,对美丽硬仆骨舌鱼的染色体进行了分析研究。结果表明,美丽硬仆骨舌鱼的二倍体染色体数目为50,核型公式为2n=2m+8sm+8st+32t,臂数(NF)为60。这一核型符合低等鱼类的基本特征,研究结果可为美丽硬仆骨舌鱼的种质标准和系统演化等提供基础数据。

Scleropages formosus is an important aquarium fish with high economic value. In order to explore its cytogenetic background, the metaphase chromosomes were observed. The phytohemagglutinin （PHA） and colchicine were injected in vivo,and kidney cells were collected and treated with low osmotic solution, and finally chromosome spread was prepared by air-drying technique. The karyotype was analyzed. The results showed that S. formosus had a diploid chromosome number of 50 and the karyotype formula was 2n=2m＋8sm＋8st＋32t, NF=60. These data will be helpful for understanding the idioplasm and systematic evolution of S. formosus.