去神经支配大鼠海马内MTSS1的表达变化

The change of MTSS1 expression in the deafferented rat hippocampus

目的:探讨切割穹窿海马伞去神经支配大鼠海马内转移抑制蛋白1(metastasis suppressor 1,MTSS1)的表达变化。方法:SD大鼠随机分成正常组和切割穹窿海马伞后1、3、5、7、14 d组,采用Real-time PCR和免疫荧光组织化学技术观察穹窿海马伞切割后海马内MTSS1 mRNA的表达变化和MTSS1阳性细胞的分布情况。结果:(1)Real-time PCR结果显示:海马内MTSS1 mRNA的相对表达量在切割后3 d开始升高(P<0.05),5 d达到最高水平(P<0.01),7 d恢复至正常水平;(2)免疫荧光组织化学染色结果显示:MTSS1阳性细胞主要表达于海马齿状回门区及颗粒下层中,切割后5 d MTSS1阳性细胞数开始增多(P<0.01),7 d继续增多并达到高峰(P<0.01),14 d时开始下降至正常水平。结论:结合本课题组以往的工作,上述结果提示切割穹窿海马伞后MTSS1的高表达可能与海马神经再生过程中神经干细胞向神经元的分化有关。

Objective： To observe metastasis suppressor 1 （MTSS1） expression in the hippocampus of deafferented rats by transected fimbria-fornix. Methods： SD rats were randomly divided into normal control group and fimbria-fornix tran- sected 1st , 3rd , 5th, 7th and 14th day group respectively. The Real-time PCR and immunofluorescence histochemistry were used to analysis the expression change of MTSS1 mRNA and the distribution of MTSSl-positive cells in hippocam- pus. Results： （ l ） Real-time PCR results showed that after the fimbria-fornix transection, the relative expression level of MTSS1 mRNA started to increase on the 3rd day（ P 〈 0.05 ） , and reached its highest level on the 5th day （ P 〈 0.01 ）. Then it declined and returned to normal levels on the 7th day. （2） Immunofluorescence histochemical results showed that MTSSl-positive cells distributed mainly in hilus and subgranular layer of dentate gyrus. The number of MTSSl-positive cells started to increase on the 5th day（ P 〈 0.01 ）, and reached its highest level on the 7th day （ P 〈 0.01 ）. Then it started to decline on the 14th day. Conclusion： The present results combined with our previous studies suggest that the up-regulated MTSS1 in the fimbria-fornix transected hippocampus may be related to neuronal differentiation of hippocam- pal neural stem cells.