聚乙二醇沉淀法在流感病毒气溶胶检测中的应用

Application of PEG precipitation method in detection of influenza virus aerosol

目的初步探讨聚乙二醇(PEG)沉淀法对流感病毒气溶胶的浓缩效果。方法于2012年12月—2013年1月随机选取南京市5家商场超市和3家综合医院,采集室内可能含流感病毒的气溶胶样品,气溶胶经过不同的吸收液浓缩量、PEG-6000和NaCl加入量进行浓缩后提取RNA,以一步法逆转录巢式PCR检测甲型流感病毒。结果当PEG-6000和NaCl终浓度为7%和2.9%(m/V)浓缩10、20 ml吸收液以及PEG-6000和NaCl终浓度为14%和5.8%(m/V)浓缩10 ml吸收液时,甲型流感病毒的阳性检出率分别为17.5%,60%,30%,差异有统计学意义(P=0.008)。结论 PEG沉淀法可应用于流感病毒气溶胶的浓缩,并利于流感病毒的检出。

Objective To explore concentrate effect of PEG-6000 precipitation for influenza virus aerosol. Methods The influenza virus aerosol was collected from five supermarkets and three general hospitals of Jiangsu in Dec., 2012 and Jan., 2013. The collection medium of influenza virus aerosol was concentrated by PEG-6000-NaC1 precipitation and then influenza virus A was detected by Nested-PCR. Results The positive rates of influenza A virus were 17.5%, 60% and 30% when the final concentration of PEG-6000/NaC1 were 7%/2.9%（m/V） for 10ml and 20ml collection medium, 14%/5.8%（m/V） for 10ml collection medium. There was significant difference in the positive rate of influenza virus in three concentrate conditions （P= 0.008）. Conclusion The PEG-6000 precipitation is applicable to the concentration of influenza virus aerosol.