Let-7与Dicer基因在肺癌组织和癌旁正常组织中表达的比较

Expression characteristics of Let-7 and Dicer gene in lung cancer

目的探讨Let-7a、Let-7b与Dicer基因在肺癌组织中的表达特征及相互调控关系。方法选择47例肺癌患者并收集癌组织和癌旁正常组织,用TRIzol法提取总RNA,实时荧光定量PCR检测基因表达量。结果 Dicer基因在癌组织中的相对表达量(9.65±2.31)明显低于癌旁正常组织(11.79±2.71),差异有统计学意义(P<0.05),Let-7a、Let-7b基因表达无明显变化。4例有远处转移的个体癌组织中Let-7b基因相对表达量(16.97±2.11)高于43例无转移者(14.87±1.92),差异有统计学意义(P<0.05)。在癌旁正常组织中,Let-7a、Let-7b均与Dicer基因的表达呈负相关(P<0.05),在癌组织中未发现相关关系。结论 Dicer基因在癌组织中表达降低,并且可能失去与Let-7a、Let-7b基因的正常调控关系。

Objective To study the expression characteristics of Let-7 and Dicer genes in lung cancer, as well as the relationship of mutual regulation. Methods Through random sampling method, 47 patients with lung cancer were selected and their cancer tissue and adjacent normal tissue were collected, TRIzol was used to extract total RNA and real-time quantitative PCR was used to detect relative gene expression levels. Results Dicer relative expression level in cancer tissue （9.65±2.31 ） was significantly lower than that in adjacent normal tissue （11.79±2.71 ）（P=0.00） and the other two genes expressions did not change significantly. Let-7b expression in the cancer tissue of individuals with metastasis （16.97 ±2.11 ） was significantly higher compared with patients without metastasis （14.87±1.92） （P=0.04）. The Dicer and Let-7a, Let-7b gene mRNA in adjacent normal tissue were negatively related （P=0.00）, but not correlated in the adjacent tissues. Conclusion Dicer expression is down-regulated in cancer tissues, and loses the normal regulation of the Let-7a, Let-7b gene.