中国被毛孢分生孢子形成过程显微结构的研究

The microstructure and formation of the conidia of Hirsutella sinensis

分生孢子是中国被毛孢(H.sinensis)生活史中极为重要的一个环节,其在冬虫夏草侵染和形成过程中有着十分关键的作用。本实验以中国被毛孢纯培养菌种为实验材料,采用显微镜观察了其菌丝体经营养生长产分生孢子过程中显微结构变化,结果表明:中国被毛孢在固体培养条件下,可以形成分生孢子,其形态和产生方式与微循环产孢过程中形成的分生孢子一致,同时,在产孢过程中,还存在瓶梗结构纵裂形成对生分生孢子的现象。

Conidia is a very important aspect in the life history of Hirsutella sinensis,and its formation plays a very crucial role in the process of Ophiocordyceps sinensis infection and formation.In this experiment,we used pure cultures of Hirsutella sinensis strains as the experimental material,and observed the microstructural changes of its mycelia during the formation of conidia using a microscope.The results showed that Hirsutella sinensis can form spores under solid culture conditions,and their morphology and the way of formation are consistent with those of the conidia formed during microcircular sporulation.Meanwhile,in the sporulation process,bottle stem structures can form on the conidia longitudinally.