盐胁迫下己唑醇铜配合物对小麦幼苗脯氨酸合成的影响

The effect of hexaconazole-Cu complex on proline accumulation in wheat seedlings under NaCl stress

以己唑醇为配体与醋酸铜配合获得元素组成异化的己唑醇铜配合物。配合物对小麦种子浸种处理,待萌发生长至幼苗阶段将其进行NaCl胁迫处理,通过对幼苗脯氨酸含量以及脯氨酸合成酶基因P5CS、P5CR表达变化的测定,评价己唑醇铜配合物对植物抗盐能力的影响。结果表明:小麦幼苗脯氨酸含量随着盐浓度的增大及胁迫时间的延长而增加,200mmol·L-1NaCl胁迫下,配合物处理后脯氨酸含量最高,达55.44μg·g-1,相比己唑醇和醋酸铜处理分别增加13.68%和58.45%;而100mmol·L-1NaCl处理6h,己唑醇铜配合物处理小麦幼苗的脯氨酸合成增幅最大,P5CS、P5CR表达量明显增加,在一定程度上缓解了小麦幼苗的渗透胁迫伤害。

The hexaconazole - Cu complex, synthesized by hexaconazole and copper acetate, was used to soak the seed of wheat to ameliorate the wheat seedlings salinity stress induced by sodium chloride （NaC1）. The effect of hexa- conazole - Cu complex on the anti-salinity stress of wheat seedlings was evaluated by proline accumulation level and the gene expression level of A1 - pyrroline - 5 - carboxylate synthetase （PSCS） and A1 - pyrroline - 5 - carboxylate reduc- tase （PCSR） of wheat seedlings. The resuhs showed that in the presence of 200 mmol· L- 1 NaCl, the proline content of the 0.03 mmol·L-1 complex treated wheat seedlings was 55.44 μg·g-l, which was 13.68% higher than that in hexa-conazole treatment, and 58.45 % higher than that in copper acetate treatment. The gene expression levels of PSCS and P5CR were quantitated by semiquantitative RT- PCR analysis. The results showed that the hexaeonazole- Cu complex enhanced the transcript levels of P5CR and P5CS. After 6 h treatment of the complex in the presence of 100 mM NaC1, the P5CS cxpress level reached the maximum, and was significantly higher than in hexaconazole treatment. It was obvious that the hexaconazole- Cu complex enhanced the proline contents, thereby played a protective role in the salinity stress of wheat seedlings.