HPLC同时测定林蛙油中α-亚麻酸、亚油酸和油酸含量

Determination of α-Linolenic Acid,Linoleic Acid and Oleic Acid in Oviductus Ranae by HPLC

目的:建立同时测定林蛙油中α-亚麻酸、亚油酸和油酸含量的HPLC方法。方法:Alltima C18色谱柱(4.6 mm×250 mm,5μm),以乙腈-0.1%磷酸(80∶20)为流动相,流速1 mL.min-1,柱温30℃,检测波长203 nm。结果:α-亚麻酸在4.76～152.16 mg.L-1(r=1),亚油酸在15.36～491.59 mg.L-1(r=0.999 8),油酸在47.28～1512.80 mg.L-1(r=0.999 9)呈良好的线性关系;平均回收率α-亚麻酸为95.8%,RSD 1.79%(n=6);亚油酸为98.6%,RSD 1.32%(n=6);油酸为99.3%,RSD 1.59%(n=6)。结论:该法操作简便,结果准确,专属性强,重复性好,可作为林蛙油质量控制的参考。

Objective： To establish a HPLC method for the determination of α-linolenic acid,linoleic acid and oleic acid in Oviductus Ranae.Method： Alltima C18（4.6 mm × 250 mm,5 μm） column was used with mobile phase of acetonitrile-0.1% phosphoric acid（80 ∶ 20）;the flow rate was 1 mL.min-1;the column temperature was kept at 30 ℃;the detection wavelength was set at 203 nm.Result： The linear ranges of αlinolenic acid,linoleic acid and oleic acid were 4.755-152.16 mg.L-1（r =1）,15.362-491.59 mg.L-1（r = 0.999 8） and 47.275-1512.8 mg.L-1（r = 0.999 9） respectively.The average recoveries were 95.8% with RSD 1.79% for α-linolenic acid,98.6% with RSD 1.32% for linoleic acid,99.3% with RSD 1.59% for oleic acid.Conclusion： The method is simple,accurate,convenient,specific and repeatable,which can be used to control the quality of α-linolenic acid,linoleic acid and oleic acid in Oviductus Ranae.