甘草的色泽与甘草苷、甘草酸含量的相关性研究

Research on the Correlation between Color Traits of Licorice and Its Content of Glycyrrhizic Acid and Liquiritin

目的:通过色差仪对甘草的颜色性状测定,对颜色特性与甘草质量评价的相关性进行探讨。方法:通过色差仪对甘草根皮和断面颜色进行测定,并应用HPLC测定甘草中指标性成分——甘草苷、甘草酸含量。结果:色差仪测定甘草颜色各色度值的RSD均<2%,结果表明色度值数据稳定性良好,方法可以使甘草性状评价指标中颜色性状指标得到量化,克服了不同人对颜色识别的主观个体差异和个体对于颜色细微差异所引起识别的不敏感性,并且数据经统计相关性分析,发现色度值与野生甘草的指标性成分成含量呈显著性相关。而色度值与栽培甘草中指标性成分含量无明显性相关性。结论:采用色差仪测定中药材及其饮片色泽,具有一定的可行性,为规范中药材质量评价体系提供了新的方法。

Objective： To explore the correlation between color traits of Licorice and its quality evaluation by colorimeter to determine the color traits of Licorice.Method： The colors of Licorice cortex and transection were determined by colorimeter.In addition,the content of the marker components of licorice,glycyrrhizic acid and liquiritin were determined by HPLC.Result： The RSD values of all colorimetric values of licorice were less than 2%,indicating a good stability of colorimetric values,and also,the method can quantify the color traits.It avoids the individual subjective discrepancy to color recognition and the insensitivity to slight difference in color.In addition,the correlation analysis showed a significant positive correlation between colorimetric values and the marker components of wild licorice,providing a basis for the traditional sensory color evaluation for the quality of Chinese drugs.However,this rule was not applicable for planting liquorices.So the traditional sensory color evaluation to the planting materials requires further studying.Conclusion： Applicating colorimeter to determine the color traits of traditional Chinese medicine is feasible,and provides a new method and reference for the traditional evaluation methods for Chinese drugs.