HPLC波长切换法测定止痛片中芍药苷与胡椒碱的含量

RP-HPLC with UV Switch Determination of Paeoniflorin and Pepper Alkali in Zhitong Tablets

目的:建立同时测定止痛片中芍药苷和胡椒碱含量的方法。方法:采用Phenomsil ODS(4.6 mm×250 mm,5μm)色谱柱,以乙腈-0.1%磷酸为流动相,梯度洗脱,流速1.0 mL.min-1,检测波长230 nm(0～15 min,芍药苷),343 nm(15～30 min,胡椒碱),柱温30℃。结果:芍药苷在4.82～48.24 mg.L-1(r=0.999 8)呈良好的线性关系,平均回收率为100.59%,RSD 1.43%(n=6),胡椒碱在2.51～25.12 mg.L-1(r=0.999 7)呈良好的线性关系,平均回收率为99.84%,RSD1.45%(n=6)。结论:方法简单,灵敏度高,可用于止痛片中芍药苷和胡椒碱的含量测定。

Objective： To establish a simultaneous determination method for paeoniflorin and pepper alkali in Zhitong Tablets.Method： The determination was performed on a Phenomsil ODS column with acetonitrile-0.1% phosphoric acid as the mobile phase.The detection wavelength was set at 230 nm（0-15 min,paeoniflorin） and 343 nm（15-30 min,pepper alkali）.The flow rate was 1.0 mL.min-1.The column temperature was maintained at 30 ℃.Result： paeoniflorin presented a good linearity in the concentration range of 4.82-48.24 mg.L-1（r = 0.999 8）.The average recovery rate was 100.59% with RSD 1.43%（n = 6）.Pepper alkali presented a good linearity in the concentration range of 2.51-25.12 mg.L-1（r =0.999 7）.The average recovery rate was 99.84% with RSD 1.45%（n = 6）.Conclusion： The method is simple,accurate and reproducible,it can be used as the basis for quality control of Zhitong Tablets.